Studies on the Artificial Seedling Production of Geoduck Clam, Panope japonica I. Spawning Induction and Hatching

코끼리조개의 인공종묘생산에 관한 연구 I. 산란유발 및 부화

  • Lee, Chae-Sung (Wast Sea Fisheries Research, Institute, National Fisheries Research & Development Institute) ;
  • Rho, Sum (Department of Aquaculture, Cheju National University) ;
  • Park, Young-Je (Kangnung Hatchery, National Fisheries Research and Development Institute)
  • 이채성 (국립수산진흥원 서해수산연구소) ;
  • 노섬 (제주대학교 증식학과) ;
  • 박영제 (국립수산진흥원 강릉수산종묘배양장)
  • Published : 1997.05.01

Abstract

In order to developed the techniques for artificial seedling production of geoduck clam, Panope japonica, various stimulution for spawning induction and hatching condition were studied. Spawning induction by the air day or UV-irradiation stimulus were not effective. Water temperature stimulus was responsed 15.0~25.0% in May and 10.0% in June. But spawning induction by the gonad incision was highest with 15.0~45.0%. Ammonium hydroxide (NH4OH) stimulus adding in seawater were responsed 15.0% at 8/1000N~10/1000N, and ammonium hyhroxide solution injected in the gonads were responsed 5.0~10.0% at 5/100N~7/100N. The highest fertilization and hatching rate at various water temperature were ranged 74.5~89.2% in 11~$17^{\circ}C, \;84.3~89.5%\;in\;8~14^{\circ}C$, respectively. the highest fertilization rate and hatching rate in various salinity were ranged 72.5~88.5% in 25~$35tetperthousand$, 82.7~86.9% in 30~$35tetperthousand$, respectively. The optimum water temperature and salinity for fertilization and hatching to the 11~14$^{\circ}C$ and 30~$35tetperthousand$, respectively.

강원연안에 서식하는 코끼리조개의 인공종묘생산 기술개발을 목적으로 산란유발 방법 및 수정과 부화를 위한 수온과 염분농도의 최적조건 등을 연구한 결과를 요약하면 다음과 같다. 코끼리조개의 산란유발은 간출자극과 자외선조사해수자극에서는 전혀 반응이 없었다. 수온자극에서는 5월에 15.0-25.0%의 반응을 보였고, 6월에는 10.0%의 반응을 보였다. 그러나 생식소를 절개한 것은 15.0-45.0%로 가장 높은 반응을 보였다. NH 하(4) OH 용액을 해수에 첨가하는 자극에서는 8/1000N-10/1000N에서 15.0%의 반응율을, 그리고 NH 하(4) OH 용액을 생식소에 주사하는 자극은 5/100N-7/100N에서 5.0-10.0%의 반응율을 보였다. 코끼리조개의 수온에 따른 수정율은 수온 11-17$^{\circ}C$범위에서 74.2-89.2%였고, 부화율은 수온 8-$14^{\circ}C$에서 84.3-90.5%로 가장 높았다. 염분 농도에 의한 수정율은 염분 25-$35tetperthousand$범위에서 72.5-88.5%, 부화율은 염분 30-$35tetperthousand$에서 82.7-86.9%였다. 수정과 부화를 위한 수온과 염분농도의 최적조건은 수온 11-$14^{\circ}C$, 염분농도 30-$35tetperthousand$로 나타났다.

Keywords

References

  1. Biol. Bull. v.105 Fertilization and artificial activation in the egg the surf clam Spisula solidissima Allen, R. D.
  2. Aquaculture v.114 Optimization of fertilization parameters for rearing surf clams (Spisula solidissima) Clotteau, G.;F. Dube
  3. Tridacna gigas. Aquaculture v.58 Spawning induction, and larval and juvenile rearing of the Giant clam Crawford, C. M.
  4. Develop. Biol. v.126 The relationships between early ionic events, the pattern of protein synthesis, and oocyte activation in the surf clam, Spisula solidissima Dube, F.
  5. Aquaculture v.40 Serotonin as an inducer of spawning in six bivalve species Gibbons, M.;M. Castagna
  6. Rep. Inst. Agr. Res. Tohoku Univ. v.87 Tank breeding of the Japanese surf clam, Mactra sachalinensis Schrenck Imai, T.(et al.)
  7. Bull. Jap. Soc. Sci. Fish. v.17 no.2 Spawning of Mytilus edulis, acid-inhibition of spawning by KCI Iwata, K. S.
  8. Bull. Tohoku Rep. Fish. Res. Lab. v.20 Artificial discharge of reproductive substance of mollusca caused by repeatedly stimulation of temperature Kanno, H.
  9. Bull. Mar. Biol. St. Jeju Nat. Univ. v.1 Ecological studies for the culture of the clam, Gomphia veneriformis.Ⅰ. Studies on the spawning season, artificial fertilization and egg development Lee, J. J.
  10. Bull. Mar. Resour. Res. Inst. Jeju Nat. Univ. v.5 The effect of irradiated seawater with ultraviolet rays on inducing spawning of some cultivated shells Lee, J. J.;J. Y. Lee
  11. Biol. Bull. v.98 Coditioning V. mercenaria for spawning in winter and breeding its larvae in the laboratory Loosanoff, V. L.;H. C. Davis
  12. Advances in Marine Biology v.1 Loosanoff, V. L.;H. C. Davis
  13. Mar. Biol. Letters v.3 Induction of spawning by serotonin in the scallop, Patinopecten yessoensis (Jay) Matsutani, T.;T. Nomura
  14. J. Exp. Biol. v.180 The effect of 5-hydroxy-tryptamine on glycolysis in the perfused ventricle of the freshwater bivalve Anodonta cygnea : evidence for posphorylation/deposphorylation control of phosphofructokinase Michaelidis, M.
  15. Science v.196 Hydrogen peroxide induces spawning in molluscs, with activation of prostaglandin endoperoxide synthetase Morse, D. E.;H. Duncan;H. Hooker;A. Morse
  16. Nippon Suisan Gakkaishi v.58 no.2 In vitro effects of estrogen and serotonin on release of eggs from the ovary of the scallop Osada, M.;K. Mori;T. Nomura
  17. Bull. Japanese Soc. Sci. Fish. v.23 no.9 Artificial discharge of reproductive elements of certain bivalves caused by tretment of seawater and by injection with $NH_4$OH Sagara, J.
  18. 江原沿岸 主要 貝類資源 調査 報告書 江原道
  19. 山口縣內海水産試驗場 v.11 ミルクイガイの種苗生産に關する硏究-I, 受精後1~2週間にみられる大量へい死について 高見東洋
  20. 山口縣內海水産試驗場 v.11 ミルクイガイの種苗生産に關する硏究-II. Up welling法による稚仔貝飼育について 高見東洋
  21. 山口縣內海水産試驗場報告 v.14 ミルクイガイの增殖に關する硏究-III, 人工種苗の沖出し時期, 大きさおよび保護網の目合と中間育成の步留りについて 高見東洋;河本良彦
  22. 山口縣內海水産試驗場硏報 v.15 ミルクイガイの增養殖に關する硏究-IV, 中間育成における潛砂床の厚さと成長, 步留り 高見東洋;河本良彦
  23. 水産增殖 v.18 ミルクイTresus keenae Kuroda et HABEの增殖に關する硏究-I, 浮游幼生と沈着初期稚貝の成長について 溝上昭男;堀田正勝
  24. 水産增殖 v.18 ミルクイTresus keenae Kuroda et HABEの增殖に關する硏究-II, 潛入稚貝の成長について 溝上昭男;堀田正勝
  25. 國立水産振興院 硏究報告 v.45 江原沿岸 코끼리조개의 棲息環境과 成長 金炯燮;朴榮濟;金完起;張貞源;金鍾斗
  26. 日水誌 v.11 no.5-6 ホタテガイ, Pecten(Pationpecten) yessoensis JAYの産卵誘發に關する試驗 木下虎一郞;澁谷三五郞;淸水二郞
  27. 香水試硏報;Bull. kagawa Pref. Fish. Exp., Sta. v.1 備讚瀨戶海域にするタイラギAkina(Servatrina)pectinata(LINNAEUS)およびミルクイTresus keenae(Kuroda et HABE)の形態的特徵 濱本俊策;高木俊祐
  28. 香水試硏報;Bull. kagawa Pref. Fish. Exp., Sta. no.1 ミルクイTresus keenae (Kuroda et HABE)の採卵適期と種苗生産上の問題點 濱本俊策;大林萬鋪
  29. 栽培技硏 v.14 no.2 備讚瀨戶鹽飽諸島海域におけるナミかイPanopea japonica(A, ADAMS)の大量發生とその漁業實態 濱本俊策;大林萬鋪
  30. 山口縣內海水産試驗場硏報 山口縣內海水産試驗場
  31. 山口縣內海水産試驗場硏報 山口縣內海水産試驗場
  32. 참담치 人工種苗生産技術에 關한 硏究 柳浩英(外)
  33. 水振年報 v.39 키조개의 種苗生産을 위한 實驗的 硏究 鄭成采;許宗秀;文榮鳳;李鐘寬;宋泉浩;金慶吉
  34. 韓國水産學會誌 v.8 no.3 대합(Meretrix Iusoria)과 가무락(Cyclina sinensis)의 初期發生 및 成長에 關한 硏究 崔信錫
  35. 水産增殖 v.14 no.3 ミルクイ幼生の水槽飼育について 龜山展志