한국양식학회지 (Journal of Aquaculture)

한국양식학회 (The Korean Society of Fisheries and Aquatic Science)
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문치가자미, Limanda yokohamae 정자의 냉장보존

Cold Storage of Sperm in Marbled Sole Limanda yokohamae

  • 발행 : 1997.11.01
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초록

A series of experiments were conducted to compare the effects of various diluents in cold storage on the marbled sole, Limanda yokohamae sperm. Various diluents of glucose, L. yokohamae serum, marine fish Ringer's solution, sodium citrate and Ca2+ free artificial seawater (ASM) (tris-HCI, pH 7.4) containing 3 mM ethylene glycol-bis (2-aminoethyl ether) tetraacetic acid (EGTA) were used to store the sperm at 4℃. The storage effect was evaluated using motility index and survival rate of sperm. Glucose and sodium citrate were found to be better diluents which maintained high motility and survival rate of sperm for a storage period of 10 days. Some morphological changes of spermatozoa were observed during the cold storage with diluents. In particular, a detachment of the nuclear enveloped and of the plasma membrane from the nucleus in spermatozoa was observed. Morphological normality of the stored spermatozoa diluted with 0.3 M glucose was better than that of the stored spermatozoa undiluted or diluted with Ca2+ free ASW containing 3 mM EGTA.

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참고문헌

  1. Nature v.172 Sperm storage and cross-fertilization of spring and autumn spawning herring Blaxer, J. H. S.
  2. Fertil. Steril. v.1 A one-minute live-dead sperm stain by means of eosin-nigrosin Blom, E.
  3. Aquaculture v.5 Study on cryogenic preservation of greymullet sperm Chao, N. H.;H. P. Chen;I. C. Liao
  4. Prog. Fish-Cult. v.28 The application of a differential staining method to low-temperature studies on goldfish spermatozoa Fribourgh, J. H.
  5. Aquaculture v.28 A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation Hara, S.;J. T. Canto;J.M. Almendras
  6. Aquaculture v.36 Chilled storage of Sarotherodon mossambicus milt. Harvey, B.;R. N. Kelley
  7. Aquaculture v.21 Controlled propagation of the African catfish, Clarias lazera II. Artificial reproduction Hogendoorn, H.;M. M. Vismans
  8. Limanda yokohamae. J. Shimonoseki Coll. Fish v.11 Effects of osmolality and $Ca^2-$ on sperm motility in marbled sole Kho, D. H.;Y. J. Chang;H. K. Lim
  9. J. Shimonoseki Coll. Fish v.11 Fundation studies in culturing of the Japanese loach, Misgurnus anguillicaudatus III. Storing of spermato-zoon Kubota, Z.
  10. Asia. Fish. Sci. v.7 Chilled storage of pikeuy bream, Acanthopagrus berda, sperm and activation in different salinities Palmer, P. J.
  11. J. Cell Biol. v.17 THe use of lead citrate at high pH as electron opaque stain in electron microscopy Reynolds, E. S.
  12. J. Fish Biol. v.17 A review of the biology, handling and storage of salmonid spermatozoa Scott, A. P.;S. M. Baynes
  13. Fish Physiology Fish gamete preservation and spermatozoan physiology Stoss, J.;Hoar, W. S.(eds.);D. J. Randali(eds.);E. M. Donaldson(eds.)
  14. Fish. Sci. v.60 Motility of pejerrey, Odontesthes bonariensis spermatozoa Strussmann, C. A.;P. Renard, H. ;F. Takashima
  15. Yoshoku v.1 Simple method for cold storage of rainbow trout spermatozoa Takahashi, K.;T. Inoda;M. Morisawa
  16. Aquaculture v.27 Preliminary observations of chilled and deep-frozen storage of grouper, Epinephelus tauvina, sperm. Withler, F. C.;L. C. Lim
  17. Prog. Fish-Cult. v.25 Physiological salines for fresh-water teleosts Wolf, B.
  18. Biostatistical Analysis Zar, J. H.