한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제26권5호
  • /
  • Pages.901-907
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  • 1997
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지

Streptozotocin 유발 당뇨쥐에서의 Cytochrome P_{450}, Xanthine Oxidase 활성과 간조직의 손상에 미치는 녹차 Catechin의 영향

Effects of Green Tea Catechin on Cytochrome $P_{450}$, Xanthine Oxidase Activities in Liver and Liver Damage in Streptozotocin induced Diabetic Rats

  • 박규영 (대구효성가톨릭대학교 식품영양학과) ;
  • 이순재 (경북실업전문대학 호텔조리과) ;
  • 임정교 (경북실업전문대학 호텔조리과)
  • 발행 : 1997.10.01
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초록

The purpose of this study was to investigate the effects of green tea catechin o n free radical generation system and peroxidative damage in the liver of streptozotocin(STZ)-induced diabetic rats. Spragu-Dawley male rats weighing 150$\pm$10gm were randomly assigned to one normal and three STZ-induced diabetic groups; diabetic groups were classified to catechin free diet(DM-oC group), 0.5% catechin diet(DM-0.5C group) and 1% catechin diet(DM-1C group) according to the levels of dietary catechin supplementation. Diabetes was experimentally induced by intravenous injection of 55mg/kg of body wt of STZ in citrate buffer(pH 4.3) after feeding of three experimental diet for 4 weeks. Animals were sacrificed at the 6th day of diabetic states. Activities of serum glutamic oxaloacetic transaminase(GPT) in DM-oC groups were higher than those of the normal group, and those in catechin supplementation group were similar to those of the normal group. Liver lipid peroxide values increased by 153%, 49%, and 27% in Dm-oC, DM-0.5C and DM-0C and Dm-1C but was not significantly different in catechin supplementation groups compared with the normal group, and liver cytochrome $P_{450}$ contents was similar to result of XOD activity. In electron microscopic examination of liver, lysosome was relatively scattered in Dm-oC and Dm-0.5C group and preserved normal shapes in DM-1C group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stress, leading to the acceleration of lipid peroxidation process, but this was reduced by anti-oxidative effect of high level of dietary catechin. It is concluded that dietary catechin serves as powerful antioxidant against lipid peroxidation in diabetic rats.

키워드

참고문헌

  1. Ann. Intern. Med. v.79 Plasma insulin and glucose profiles in normal, obese and diabetic persons Gonuth, S. M.
  2. Diabetes v.31 Metabolism of cholesterol and plasma triglycerides in nonketotic diabetes mellitus Abrams, J. J.;Ginsberg, H.;Grundy, S. M.
  3. JAMA v.241 Diabetes and cardiovascular disease Kannel, W. B.;McGee, D. L.
  4. Am. J Med. v.66 Lipid metabolism in diabetes mellitus Saudek, C. D.;Eder, H. A.
  5. Diabetes v.23 Morbidity in mortality in diabetics in the Framingham population Garcia, M. J.;Mcnamara, P. M.;Gordon, T.;Kannell, W. B.
  6. N. Eng. J Med. v.320 Mjodification of low-density-lipoprotein that increase its atherogenicity Steinberg, D.;Carew, T. E.;Khoo, J. C.;Witztum, J. L.
  7. Lipids v.26 Vitamin E and the susceptibility of erythrocytes and reconstituted liposomes to oxidative stress in aged diabetics Urano, S.;Midori, H.;Tochihi, N.;Matsuo, M.;Shiraki, M.;Ito, H.
  8. Exper. Gerontol. v.25 Oxidative stress as a cousal factor indifferentiation and aging. A unifying hypothesis Sohal, R. S.;Allen, R. C.
  9. Metabolism v.40 Vitamin C and vitamin E status in the spontaneously diabetic BB rat before the onset of diabetes Behrens, W. A.;Madeae, R.
  10. Diabetes v.35 Triglyceride-lowering effects of dietary vitamin E in streptozotocin-induced diabetic rats in Creased lipoprotein lipase activity in livers of diabetic rats fed high dietary vitamin E Prichard, K. A.;Patel, S. T.;Karper, C W.;Newman, H. A.;Panganamala, R. V.
  11. Life Sci. v.33 The lack of effectiveness of (-) epicatiechin against alloxan induced diabetes in wistar rats Sheehan, E. E.;Stiff, D. D.;Duah, T.;Slatkin, D. J.
  12. 茶藥硏究報告 v.71 茶の分析法 池ケ俗賢次郞;高柳博次;何南農正
  13. Am. J. Clin. Pathol. v.28 A colorimetric method for the determination of serum glutamin oxaloacetic and glutamic pyruvic transaminase Reitman, S;Frankel, S.
  14. Clinica. Chemica. Acta v.90 Serum lipid peroxide in cerebrovascular disorders determined by a new colormetric method Satoh, K.
  15. 한국식품영양과학회지 v.25 식이 vitamin E가 Streptozotocin 유발 당뇨쥐의 간장 및 폐조직에서의 microsomal mixed function oxidase system에 미치는 영향 박영란;이순재;임영숙;주길재
  16. J. Biol. Chem. v.244 The regulation of rat liver xanthine oxidase Stripe, F.;Della, C. E
  17. J. Biol. Chem. v.239 The carbon monoxide binding pigment of liver microsomes. Ⅱ. Solubilization, purification, and propertics Omura, T.;Sato, R.
  18. Am. J. Pathol. v.93 Improvement of epoxy resin embedding method Luft, J. H.
  19. J. Cell. Biol. v.17 The use of lead citrate at high pH as an electron opaque stain in electron microscopy Reynolds, E. S.
  20. J. Biochem v.131 Characterization of alternative molecular forms of xanthine oxidase in the mouse Duke, E. J.;Joyce, P.;Ryan, J. P.
  21. Infection and Immunity v.3 Induction of xanthine oxidase by virus infections in newborn mice Ziegler, D. W.;Hutchison, H. D.;Kissing, R. E.
  22. 한국영양학회지 v.22 한국산 녹차로부터 분리한 축합형 탄닌의 xanthine oxidase 저해효과 조영제;천성숙;최청
  23. J. Natural Products v.51 Inhibition of cow's milk xanthine oxidase by flavonoids Hayashi, T.;Sawa, K.;Morita, N
  24. Planta Medica. v.57 Inhibitory effects of galloylated flavonoids on xanthine oxidase Hatano, T.;Yasuhara, T.;Yoshihara, R.;Okuda, Y.
  25. J. Biochem. Toxicol. Modulation of the rat hepatic cytochrome $P_{450}$ composition by long-term streptozotocin-induced insulin-dependent diabetes Barnett, C. R.;Flatt, P. R.
  26. 한국영양학회지 v.22 어유 및 과산화유를 섭취한 흰주에 있어서 플라보노이드 (+)-카테킨의 산화안정 효과 권미나;최재수;변대석
  27. Naunyn-Schmiedeberg's Arch. Pharmacol. v.312 Protective effect of (+)-cyanidanol-3 in acute liver injury induced by galatosamine or carbon tetrachloride in rats Perrissoud, D.;Weibel, I.