Preventive Nutrition and Food Science
- Volume 2 Issue 4
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- Pages.354-359
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- 1997
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- 2287-1098(pISSN)
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- 2287-8602(eISSN)
Production and Characterization of Phenylalanine Ammonia-lyase from Rhodotorula aurantiaca K-505
- Cho, Dae-Haeng (Department of Chemical Engineering, The University of Seoul) ;
- Chae, Hee-Jeong (R&D Center, Daesang Corp., Incheon) ;
- Kim, Eui-Yong (Department of Chemical Engineering, The University of Seoul)
- Published : 1997.12.01
Abstract
Optimal cultivation conditions for the production of phenylalanine ammonia-lyase(PAL) from Rhodotorula aurantiaca K-505 were selected, and the kinetic parameters of the produced PAL were determined. The most suitable carbon and nitrogen sources were glucose and tryptone, respectively. The strain expressed PAL constituttively when using the optimized semi-complex media. High cell density culture could be critical for maximal production of PAl since the PAL ynthesis was growth associated. maximum PAL activity was observed at initial pH 6.0. although the ll growth was not markedly affected by temperature between 22 and 28
Keywords
- Phenylalanine ammonia-lyase(PAL);
- Rhodotorula aurantiaca;
- phenylalanine;
- culture conditions;
- kinetic parameters