Korean Journal of Veterinary Pathology (한국수의병리학회지)

Korean Society of Veterinary Pathology (한국수의병리학회)
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Digoxigenin-labeled in situ Hybridization for Diagnosis of Aujeszky's disease in Pigs

Digoxigenin-labeled in situ Hybridization을 이용한 오제스키병의 진단

  • Kim, Soon-Bok (Institute of Animal Medicine, College of Veterinary medicine, Gyeongsang National University) ;
  • Moon, Oun-Kyung (National Veterinary Research Institute) ;
  • Song, Geun-Suk (Yoohan Pharmaseutical Co.) ;
  • Yeo, Sang-Geon (Institute of Animal Medicine, College of Veterinary medicine, Gyeongsang National University)
  • 김순복 (경상대학교 수의과대학 동물의학연구소) ;
  • 문운경 (수의과학연구소) ;
  • 송근석 (유한양행) ;
  • 여상건 (경상대학교 수의과대학 동물의학연구소)
  • Published : 1997.12.01
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Abstract

The objective of this study was to develop digoxigenin (DIG)-labeled in situ hybridization (ISH) test for diagnosis of Aujeszky's Disease(AD) in infected organs. Specific DNA with well conserved gene sequences encoding gp50 antigen in AD virus (ADV) was obtained by Polymerase Chain Reaction (PCR) method. A pair of oligonucleotide primers used in PCR allowed amplification of a 217 bp sequence from the gp50 ADV gene. The DNA was then labeled with DIG by primer labeling method for use as probe in ISH test to detect ADV nucleic acids in various tissue. Positive hybridization was demonstrated by dark pigmentation in nuclei and cytoplasm of ADV infected cells particularly in brain tonsillar crypt epithelium and pulmonary alveolar cells. This result suggests that ISH is a valuable sensitive and rapid diagnostic test for AD.

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