한국수의병리학회지 (Korean Journal of Veterinary Pathology)
- 제1권1호
- /
- Pages.33-39
- /
- 1997
- /
- 1226-766X(pISSN)
Pathological Changes in Rats Fed petasites japonicus Maxim II. Immunohistochemical Localization of Cytochrome P4502E1 and GST-P in Liver
- Jee, Young-Heun (College of Veterinary Medicine Kyungpook National University) ;
-
Lee, Cha-Soo
(College of Veterinary Medicine Kyungpook National University) ;
- Jeong, Kyu-Shik (Korea Research Institute of Bioscience & Biotechnology, KIST)
- 발행 : 1997.04.01
초록
We investigated metabolism and carcinogenesis in livers of Sprague-Dawley rats fed juices and pelleted diets containing Korean native plants petasites japonicus Maxim by evaluating cell localization and expression of cytochrome P450s and GST-P. Anti-cytochrome P450s application in liver sections revealed three to four times increased expression of cytochrome P450E1 immunoreactivity in degenerative hepatocytes when compared to histologically normal hepatocytes. Anti-GST-P in showed positive pren plastic foci as well as in individual hepatocytes randomly scattered throughout all liver sections examined. Additionally GST-P was evident in proliferative endothelial cells and biliary epithelial cells in exposed rat livers. These results suggested that the increased level of cytochrome P4502E1 in affected hepatocytes was a direct consequence of Petasites japonicus toxicity. Further immunoreactivity to anti-GST-P in hepatocytes endothelial cells and biliary epithelial cells indicated a possible preneoplastic effects of Petasites japonicus in Sprague-Dawley rat.
키워드