한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제25권6호
  • /
  • Pages.571-579
  • /
  • 1997
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

알칼리 내성 Bacillus sp. YA-14 유래의 중복 Promotor를 이용한 재조합 Plasmid로부터의 Pectate Iyase의 발현

  • 박희경 (연세대학교 생명공학과.생물산업소재 연구센터) ;
  • 함병권 (단국대학교 식품공학과.연세대학교 생물산업소재 연구센터) ;
  • 유주현 (연세대학교 생명공학과.생물산업소재 연구센터) ;
  • 배동훈 (단국대학교 식품공학과.연세대학교 생물산업소재 연구센터)
  • Park, Hee-Kyoung (Department of Biotechnology and Bioproduct Research Center, Yonsei University) ;
  • Hahm, Byoung-Kwon (Department of Food engineering, Dankook University, Korea and Bioproduct Research Center Yonsei University) ;
  • Yu, Ju-Hyun (Department of Biotechnology and Bioproduct Research Center, Yonsei University) ;
  • Bai, Dong-Hoon (Department of Food engineering, Dankook University, Korea and Bioproduct Research Center Yonsei University)
  • 발행 : 1997.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

For the overproduction of pectate lyase (PL), the recombinant plasmid pl2BS fl which has strong promoter from alkali-tolerent Bacillus sp. YA-14 was used. In order to overexpress the pectate lyase by the action of overlapping strong promoter in pl2BS$\Delta$fl, 1.6 kb of PL gene was inserted into pl2BS$\Delta$fl to form pl2BS$\delta$f1-PL and the enzyme was expressed. But decreased expression efficiency of the PL gene was observed and it was due to the presence of the transcription terminator region on the upstream of the PL gene. The transcription terminator of the PL gene in pl2BS$\delta$f1-PL was removed and the resulting plasmid p12BS$\Delta$fl$\Delta$PL was formed. Bacillus subtilis 207-25 harboring the recombinant plasmid, p12BS$\Delta$fl$\Delta$PL, revealed increased expression efficiency with chloramphenicol induction when cat-86 was used as a reporter gene.

키워드

참고문헌

  1. Cloning vectors v.2 Pouwels,P.H.;B.E.E.Valk;W.J.Brammer
  2. Bacillus Molecular Genetics and Biotechnology Applications Ganesan,A.T.;J.A.Hoch
  3. J. Biol. Chem. v.258 Transcriptional and translational start sites for the Bacillus thuringiensis crystal protein gene Wong,H.C.;H.E.Schnept;H.R.Whiteley
  4. Nucleic Acids Res. v.11 DNA sequence of the tandom ribosomal RNA promoter for B. subtilis operon rrnB Stewart,G.C.;K.F.Bott
  5. Nucleic Acids Res. v.11 Structure and organization of rRNA operons in their region of the replication origin of Bacillis subtilis chromosome Ogasawara,N.;S.Moriya;H.Yoshikawa
  6. Nature v.302 Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene Johnson,W.C.;C.P.Moran Jr.;R.Losick
  7. Cell v.5 Promoter for a developmental regulated gene in Bacillus subtilis Moran,Jr.C.P.;N.Lang;C.D.B.Banner;W.G.Haldenwang;R.Losick
  8. Mol. Gen. Genet. v.207 Promoter switching during development and the termination site of the σ⁴³ operon of Bacillis subtilis Wang,L.F.;R.H.Doi.
  9. J.Bacteriol. v.160 Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases Kawamura,F.;R.H.Doi.
  10. J.Bacteriol. v.146 Cloning restriction fragments that promote expression of a gene in Bacillus subtilis Williams,D.H.;E.J.Duvoll;P.S.Lovett.
  11. J.Bacteriol. v.155 Restriction fragments that exert promoter activity during postexponential growth of Bacillus subtilis Mongkolsuk,S.;P.S.Lovett.
  12. Kor. J. Appl. Microbiol. Biotechnol. v.19 Studies on the properties of the promoter from alkali-tolerant Bacillus sp. Par,H.K.;Y.S.Park;J.M.Kim;J.H.Yu.
  13. Kor. J. Appl. Microbiol. and Bioeng. v.16 Cloning of pectate lyase gene of alkali-tolerant Bacillus sp. YA-14 and its expression in Escherichia coli Yu,J.H.;Y.S.Park;J.M.Kim;I.S.Kong;Y.J.Chung
  14. Nucleic Acids Res. v.9 Rapid and efficient cosmid cloning Ish-Horowicz,D.;J.F.Burke
  15. Molecular cloning: A Laboratory Maual Maniatis,T.;E.F.Fritsch;J.Sambrook
  16. Mol. Gen. Genet. v.168 High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA Chang,S.;S.W.Cohen
  17. J.Bacteriol. v.159 Molecular cloning of pectate lyase genes from Erwinia chrysanthemi and their expression in Escherichia coli Keen,N.T.;D.Dahlbeck;B.Staskawicz;W.Belser
  18. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford,H.B.
  19. Method in Enzymol v.43 Chloramphenicol acetyltransferase from the chloramphenicol-resistant bacteria Shaw,W.V.
  20. Method in Enzymol v.153 Plasmids for the selection and analysis of prokaryotic promoters Brosius,J.
  21. Mol. Gen. Genet. v.199 A transcription termination signal immediately precedes the coding sequence for the chloramphenicol-in-ducible plasmid gene cat-86 Ambulos,N.P.Jr.;S.Mongkolsuk;P.S.Lovett
  22. J.Bacteriol. v.157 Translational coupling of the trpB and trpA genes in the Escherichia coli tryptophan operon Aksoy,S.;C.L.Squires;C.Squires
  23. J. Microbiol. Biotech. v.3 Structure and regulation of a complex promoter region from an alkali-tolerant Bacillus sp. Kim,J.M.;Y.S.Park;H.K.Park;D.Y.Yum;D.H.Bai;J.H.Yu.