Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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사과 겹무늬썩음병균에 대한 Bacillus sp. SS279의 항진균활성과 생물학적 방제

  • Kim, Sam-Sun (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Joo, Gil-Jae (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Uhm, Jae-Youl (Department of Agricultural Biology, College of Agriculture, Kyungpook National University) ;
  • Kim, Young-Jae (Department of Microbiology, Changwon National University) ;
  • Rhee, In-Koo (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University)
  • Published : 1997.10.01
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Abstract

From apple skin, we isolated a bacterial strain which strongly inhibited the growth of apple white rot fungus, Botryosphaeria dothidea. The isolated strain, designated as SS279, was identified to be the genus Bacillus. The antifungal activity of Bacillus sp. SS279 was found in the culture filtrate. The production of antifungal substances occurred during logarithmic phase and was the highest when cultures reached the stationary growth phase. The optimum ranges of temperature and pH for its production were 25-30$\circ$C and 4.5-9.0, respectively. The culture filtrate of Bacillus sp. SS279 exhibited a strong inhibitory effect on the spore germination and germ tube elongation of B. dothidea. Autoclaved culture filtrate of Bacillus sp. SS279 showed only a slight decrease in antifungal activity, indicating that the Bacillus sp. SS279 produce heat-stable antifungal substances. In in vivo bioassay, Bacillus sp. SS279 also showed antagonistic activity against apple white rot caused by B. dothidea.

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References

  1. Plant Dis v.72 Spore release and infection periods of Botryosphaeria dothidea on Blurry in North Carolina Creswell, T. C.;R. D. Milholland
  2. 농촌진흥청 산합협동 연구 보고서 v.23 사과 부패병균의 동정 및 전염 경로에 관한 연구 김종천
  3. 植物防疫 v.38 リンゴ輪紋病の 發生生態と 防除 林重昭
  4. Phytopathology v.71 Production and dispersal of ascospores and conidia of Physalospora obtusa and Botryosphaeria dothidea in apple orchards Sutton, T. B.
  5. Plant Dis v.67 Inoculum availability and pathogenic variation in Botryosphaeria dothidea in apple production areas of North Calorina Sutton, T. B.;J. V. Boyne
  6. Compendium of Apple and Pear Diseases White rot Sutton, T. B.;A. L. Jones(ed.);H. S. Aldwinckle(ed.)
  7. 경북 대학교 농업과학기술연구소 세미나 특집호 사과 겹무늬썩음병의 병원균, 발생요인 및 방제대책 이두형
  8. 경상북도 용역연구 보고서 미국의 식물검역기준에 적합한 사과병해 방제체계 연구 엄재열
  9. J. Phytopathology v.134 An in vivo screening method for antifungal activity against the plant pathogen Pythium ultimum Rosendahl, C. N.;L. W. Oison
  10. J. Phytopathol v.134 Identification of antagonists for biological control of the post harvest pathogens Pezicula malicorticis and Nectria galligena on apple Schiewe, A.;K. Mendgen
  11. 영남대학교 응용미생물학과 석사학위논문 생물방제균 Bacillus subtilis YB70이 생산하는 항진균성 항생물질 및 유전적 육종 김용수
  12. Bergey's manual of Systematic bacteriology v.2 Sneath, P. H. A.;N. S. Mair;M. E. Sharpe;J. G. Holt
  13. Methods for General and Molecular Bacteriology Phenotypic characterization Smibert, R. M.;N. R. Krieg;Philipp Gerhardt(et al)(ed)
  14. Phytopathology v.112 Phenolics compounds in stems of sunflower plants inoculated with Sclerotinia sclerotiorum and inhibitory effect on the fungus Bazzalo, M. E.;E. M. Heber;M. A. Delperomatinez
  15. Biocontrol of Plant Diseases v.1 Trichoderma as a biocontrol agent Wells, H. D.;K. G. Kukerji(ed.);K. L. Garg(ed.)
  16. Appl. Environ. Microbiol. v.57 Pseudomonas stutzeri YPL-1 genetic transformation and antifungal mechanism against Fusarium solani, an agent of plant root rot Lim, H. S.;Y. S. Kim;S. D. Kim
  17. Bacillus subtilis and Other Gram-Positive Bacteria Peptide antibiotics Zuber, P.;M. M. Nakano;M. A. Marahiel;A. L. Sonenshein(et al)(ed)