Candida tropicalis에 의한 Xylose 와 Glucose로부터 Xylitol 생산

  • 오덕근 (우석대학교 식품공학과 Tel. 82-652-290-1632 E-mail : deokkun@unitel.co.kr) ;
  • 김상용 (동양제과(주) 기술개발연구소)
  • Published : 1997.10.01

Abstract

Xylitol production from xylose and glucose was investigated using Candida tropicalis KFCC-10960. As glucose concentration in xylose medium was increased, ethanol production increased. However, xylitol production was maximum at glucose concentration of 10 g/l. The concentrated cells grown on xylose or glucose were inoculated in xylose medium. The specific activities of xylose reductase and xylitol dehydrogenase, and xylitol production in concentrated cells grown on glucose were the same as those in concentrated cells grown on xylose. The results suggested that cells grown on glucose had the same xylitol producing activity as those grown on xylose. By feeding glucose in xylose medium, cell growth was achieved from glucose and xylitol production was obtained from xylose. By using this technique, a final xylitol concentration of 261 g/l was achieved from 300 g/l xylose in 41 hours which corresponded to a xylitol yield from xylose of 87% and a xylitol productivity of 6.37 g/1-h.

Keywords

References

  1. J. Food Sci. v.45 A quantitative study of mannitol, sorbitol, xylitol, and xylose in wild berries and commercial fruits Makinen, K. K.;E. Soderling
  2. Food Technol v.42 Xylitol in sugar-free confections Pepper, T.;P. M. Olinger
  3. Food Technol v.32 Xylitol: Its properties and food applications Modi, A.
  4. Adv. Food. Res. v.28 Food technological evaluation of xylitol Hyvonen, L.;P. Koivistoinen
  5. Biotechnol. Bioeng. v.25 Conversion of pentoses by yeasts Gong, C. S.;T. A. Claypool;L. D. McCracken;C. M. Maun;P. P. Ueng;G. T. Tsao
  6. Biotechnol. Bioeng. v.40 Production of xylitol from D-xylose by Candida tropicalis: Optimization of production rate Horitsu, H.;Y. Yahashi;K. Takamizawa;K. Kawai;T. Suzuki;N. Watanabe
  7. Biotechnol. Lett v.13 Xylitol production by Candida guillermondii: Fermentation behavior Meyrial, V.;J. P. Delgenes;R. Moletta;J. M. Navarro
  8. J. Ferment. Bioeng. v.67 Conversion of D-xylose into xylitol by immobilized cells of Candida pelliculosa and Methanobacterium sp. HU Nishio, N.;K. Sugawa;N. Hayase;S. Nagai
  9. Biotechnol. Lett v.11 D-xylose fermentation by Candida shehatae Pichia stipitis at low dissolved oxygen levels in fed-batch cultures Du Preez, J. C.;B. van Driessel;B. A. Prior
  10. J. Indust. Microbiol. v.9 Cultivation of Candida blankii in simulated bagasse hemicellulose hydrolysate Meyer, P. S.;J. C. Du Preez;S. Kilian
  11. Enzyme Microb. Technol. v.16 Biochemistry and physiology of xylose fermentation by yeasts Hahn-Hagerdal, B.;H. Jeppsson;K. Skoog;B. A. Prior
  12. Appl. Microbiol. v.18 Microbial production of xylitol from glucose Onishi, H.;T. Suzuki
  13. Biotechnol. Lett v.12 Simultaneous fermentation of D-xylose and glucose by Candida shehatae Kastner, J. R.;R. S. Roberts
  14. 산업미생물학회지 v.25 Candida parapsilosis DS-72에 의한 xylose로 부터 xylitol의 생산 오덕근;김상용
  15. Meth. Enz. v.9 D-xylose reductase and D-xylitol dehydrogenase from Penicillium chrysogenum Chiang, C.;S. G. Knight
  16. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry, O. H.;N. J. Rosebrough;L. Farr;R. J. Randall
  17. Biotechnol. Lett v.4 Demonstration of D-xylose reductase and D-xylitol dehydrogenase in Pachsolen tannophilus Smiley, K. L.;P. L. Bolen
  18. 산업미생물학회지 v.24 Candida parapsilosis에 의한 xylitol 생성시 포도당의 영향 오덕근;김종화
  19. 한국식품과학회지 v.28 Candida parapsilosis에 의한 xylitol 생산시 xylose와 glucose의 영향 오덕근;김상용
  20. J. Ferment. Bioeng. v.83 Improvement of xylitol production by controlling oxygen supply in Candida parapsilosis Kim, S. Y.;J. H. Kim;D. K. Oh
  21. Biotechnol. Lett. v.11 D-xylose fermentation by Candida shehatae and Pichia stipitis at low dissolved oxygen levels in fed-batch cultures Du Preez, J. C.;B. van Driessel;B. A. Prior
  22. Biotechnol. Lett v.13 Study of xylitol formation under oxygen limiting conditions Furlan, S. A.;P. Bouilloud;P. Strehaiano;J. P. Riba
  23. J. Ferment. Bioeng. v.81 Production of xylitol from D-xylose by Candida tropicals: The effect of D-glucose feeding Yahashi, Y.;H. Horitsu;K. Kawai;T. Suzuki;K. Takamizawa