한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제25권5호
  • /
  • Pages.459-463
  • /
  • 1997
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

페놀 분해 Rhodococcus sp. DGUM 2011의 분리 및 특성

  • 오정석 (동국대학교 대학원 응용생물학과) ;
  • 한영환 (동국대학교 자연과학대학 생물학과 Tel. 82-561-770-2213 E-mail : yhhan＠mail.dongguk.ac.kr)
  • 발행 : 1997.10.01
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초록

A bacterium DGUM 2011 has been selected from various samples of industrial wastewater and soil. Based on the morphological and physiological characteristics, the isolate DGUM 2011 was identified as Rhodococcus sp. and named as Rhodococcus sp. DGUM 2011. The optimal temperature and pH for the cell growth of Rhodococcus sp. DGUM 2011 were 37$\circ$C and 7.6, respectively. When phenol was added to the minimal media as a sole source of carbon and energy, the concentrations of maximum and optimum for cell growth was 0.10% and 0.08%, respectively. When 0.05% phenol was given in the minimal media, Rhodococcus sp. DGUM 2011 completely utilize it within 24 hrs. The isolate could utilize benzoic acid, p-hydroxybenzoate, p-cresol, tyrosine and phloroglucinol. The isolate possessed both catechol 1,2-dioxygenase and 2,3-dioxygenase activity. However, the activity of catechol 1,2-dioxygenase was much higher than that of 2,3-dioxygenase, which suggests that the isolate might degrade phenol via both ortho- and meta-cleavage, mainly via ortho-cleavage.

키워드

참고문헌

  1. J. Bacteriol. v.169 Dechlorination and para-hydroxylation of polychlorinated phenols by Rhodococcus chlorophenolicus Apajalahti, J. H. A.;M. S. Salkinoja-Salonen
  2. Appl. Microbiol. Biotechnol. v.35 Biodegradation of 4-chlorophenol by adsorptive immobilized Alcaligenes sp. A 7-2 in soil Balfanz, J.;H. J. Rehm
  3. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  4. J. Bacteriol. v.124 Metabolism of phenol and cresols by Bacillus stearothermophilus Buswell, J. A.
  5. Appl. Environ. Microbiol. v.56 Phenol and trichloroethylene degradation by P. cepacia G4: Kinetics and interactions between substrates Falson, B. R.;P. J. Chapman;P. H. Pritchard
  6. Methods for General and Molecular Bacteriology Gerhardt, P.;R. G. E. Murray;W. A. Wood;N. R. Krieg
  7. Bergey's Manual of Systematic Bacteriology v.2 Genus Rhhodococcus Goodfellow, M.;P. H. A. Sneath(ed.);N. S. Nicholas(ed.);M. E. Sharpe(ed.);J. G. Holt(ed.)
  8. Appl. Microbiol. Biotechnol. v.37 Degradation of phenol and phenolic compounds by Pseudomonas putida EKII Hinteregger, C.;R. Leitner;M. Loidl;A. Fersch;F. Streichsbier
  9. J. Bacteriol. v.107 Dissimilation of aromatic compounds by Alcaligenes eutrophus Johnson, B. F.;R. Y. Stanier
  10. Environ. Scien. and Tech. v.13 Priority pollutants Keith, L. H.;W. A. Telliard
  11. Kor. J. Microbiol. v.25 Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbons Kim, J. W.;C. K. Kim;Y. C. Kim;J. H. Yeoum;J. G. Lee
  12. Kor. J. Microbiol. v.32 Partial purification of catechol 1, 2-dioxygenase from Pseudomonas putida SM25 and production of cis,cis-muconic acid by the purified enzyme Kim, J. Y.;S. T. Kim;S. O. Kang;K. H. Min
  13. Bergey's Manual of Systematic Bacteriology Krieg, N. R.(ed.);J. G. Holt(ed.)
  14. Kor. J. Appl. Microbiol. Biotechnol. v.22 Isolation and characterization of a phenol-degrading strain, Acinetobacter sp. GEM2 Lee, C. H.;H. M. Oh;T. J. Kwon;G. S. Kwon;S. G. Lee;H. H. Suh;B. D. Yoon
  15. Biotechnol. Lett. v.11 Kinetics and fluorometric behaviour of a phenol fermentation Li, J. K.;A. E. Humphrey
  16. Biotechnol. Lett. v.9 Selection and adaptation of a phenol-degrading strain of Pseudomonas Masque, C.;M. Nolla;A. Bordons
  17. Appl. Microbiol. Biotechnol. v.33 Degradation of phenol by mixed culture Morsen, A.;H. J. Rehm
  18. Methods in Enzymology v.17A Metapyrocatechase(Pseudomonas) Nozaki, M.
  19. Bioresource Technol v.51 Continuous degradation of phenol by Rhodococcus sp. immobilized on granular activated carbon and in calcium alginate Pai, S. L.;Y. L. Hsu;N. M. Chong;C. S. Sheu;C. H. Chen
  20. Bergey's Manual of Systematic Bacteriology v.1 Genus Pseudomonas Palleroni, N. J.;N. R. Krieg(ed.);J. G. Holt(ed.)
  21. Appl. Environ. Microbiol. v.55 Monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in P. putida F1 Spain, J. GC.;G. J. Zylstra;C. K. Blake;D. T. Gibson