한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제25권3호
  • /
  • Pages.335-337
  • /
  • 1997
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

MTT 방법에 의한 항진균성 활성효과의 측정

  • 이동건 (한국과학기술연구원 생명공학연구소 펩타이드공학연구그룹) ;
  • 이성구 (한국과학기술연구원 생명공학연구소 펩타이드공학연구그룹) ;
  • 김길룡 (한국과학기술연구원 생명공학연구소 펩타이드공학연구그룹) ;
  • 함경수 (한국과학기술연구원 생명공학연구소 펩타이드공학연구그룹)
  • 발행 : 1997.06.01
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초록

In this study, we show a convenient MTT assay for detect the susceptibility of yeast-like form of Trichosporon beigelii against antifungal agents. This assay was developed based on mitocondrial respiration by determining reduction of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) to formazan. Cells of T beigelii are seeded into 96-well microtiter plates, and antifungal agents, amphotericin B, magainin and CA-ME hybrid peptide were added with various concentrations. After 24 hr incubation, MTT was added, then incubations were continued for 4 hr. Formazan formation was quantified photometrically after extraction of the formazan with acid sodium dodesyl sulfate (SDS). From this assay, we could obtained MICs of antifungal agents against T. beigelii. The presented method can easily be used as an effective methods to assess the antiftingal action of various agents on yeasts with minimal amounts of antifungal agents.

키워드

참고문헌

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