Lactobacillus jensenii YW-33이 생산하는 다당류성 생물응집제 및 생산조건

  • 서호찬 (고려대학교 생명공학원) ;
  • 최양문 (고려대학교 생물공학연구소) ;
  • 조홍연 (고려대학교 생명공학원) ;
  • 양한철 (고려대학교 생명공학원)
  • Published : 1997.06.01

Abstract

A bioflocculant producing bacterium for wastewater treatment was isolated and identified as Lactobacillus jensenii. The bioflocculant was supposed as a polysaccharide. Lactobacillus jensenii YW-33 produced the flocculant with highest activity in the medium composed of 2% sucrose, 0.05% tryptone, 0.5% yeast extract, 0.01% K$_{2}$HPO$_{4}$, 0.01% KH$_{2}$PO$_{4}$, 0.01% NaCl, 0.005% MnSO$_{4}$, 5H$_{2}$O and 0.2% Tween 80. The optimal culture pH and temperature were 7.0 and 25$\circ$C, respectively. In the time course of production with jar fermentor, the productivity of the flocculant was increased in proportion to the cell growth and the cultivation time for maximum production was 24 hrs, showing flocculating activity of 1,130 units per ml.

Keywords

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