Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Cyclodextrin Glucanotransferase의 고정화와 당전이 스테비오사이드 제조에 관련된 반응 특성

  • Published : 1997.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

For the continuous production of transglucosylated steviosides, cyclodextrin glucanotransferase from Bacillus macerans was immobilized onto Diaion HPA 75 (styrene-divinylbenzene resin) that was screened from ion exchange resins, synthetic adsorbents and chitosan derivatives. The parameters influencing enzyme immobilization were examined in order to maximize the activity of immobilized enzyme. The optimum conditions for immobilization turned out to be: contact time 2 hr at 30$circ$C, pH 6$sim$9, and enzyme loading 20mg protein/g resin at 4.4 Os/Kg as osmolarity. Competing with other molecules having low molecular weight, enzyme was immobilized reversibly. The activity of immobilized enzyme was as high as 180U/g resin when the diafiltrated solution of stock enzyme was used. The optimum conditions for transglucosylation were as follows: pH 6.0, temperature 50$circ$C, 30% substrate solution composed of 15% stevioside mixture and 15% dextrin of which value of dextrose equivalent was about 9.0.

Keywords

References

  1. BIO INDUSTRY v.7 ステピオ-ル配糖體の酵素工學的甘味改良 田中 治;大谷利弘
  2. Agric. Biol. Chem. v.53 Study on improvement of sweetness of steviol bisglycosides: selective enzymice transglucosylation of the 13-O-glycosyl moiety Mizutani, K.;T. Miyata;R. Kasai;O. Tanaka;S. Ogawa;S. Doi
  3. U.S. Patent 4219571 Process for producing a sweetner Miyake, T.
  4. Agric. Biol. Chem. v.53 Production of rubusoside derivatives by transgalactosylation of vavious β-galactosidases Kitahata, S.;H. Ishikawa;T. Miyata;O. Tanaka
  5. Agric. Biol. Chem. v.54 Production of stevioside and rubusoside derivatives by transfructosylation of β-fructofuranosidase Ishikawa, H.;S. Kitahata;K. Ohtani;C. Ikuhara;O. Tanaka
  6. Agric. Biol. Chem. v.53 Production of rubusoside derivatives by transgalactosylation of vavious α-galactosidases Kitahata, S.;H. Ishikawa;T. Miyata;O. Tanaka
  7. Agric. Biol. Chem. v.53 Enzymic transglucosylation products of stevioside: separation and sweetness-evaluation Fukunaga, Y.;T. Miyata;N. Nakayasu;K. Mizutani;R. Kasai;O. Tanaka
  8. Carbohydrate Res. v.61 Purification and some properties of Bacillus macerans cycloamylose(cyclodextrin) glucanotransferase Kobayashi, S.;K. Kainuma;S. Suzuki
  9. J. Jpn. Soc. Starch Sci. v.33 The continuous reaction of cyclodextrins formation by the clumn method using the immobilized enzyme on ion exchange resins Hashimoto, H.;K. Hara;N. Kuwahara;S. Sakai;N. Yamamoto
  10. J. Chem. Tech. Biotechnol. v.48 A novel method for continuous production of cyclodextrins using an immobilized enzyme system Su, C.;C. Yang
  11. 한국산업미생물학회지 v.22 생전분을 당공여체로 한 분쇄마찰매체 함유 효소반응계에서의 stevioside의 당전이 반응 기작 백승걸;박동찬;허태린;이용현
  12. 한국생물공학회지 v.9 생전분을 당공여체로 한 stevioside의 당전이 반응의 동력학적 해석 박동찬;백승걸;이용현
  13. J. Ferment. Bioeng. v.82 An empirical rate equation for the fructooligosaccharides-producing reaction catalyzed by β-fructofuranosidase Kim, M. H.;M. J. In;H. J. Cha;Y. J. Yoo
  14. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  15. 한국농화학회지 v.39 변이주 Bacillus sp. A 4442가 생산하는 갈락토스 전이활성이 높은 β-galactosidase의 고정화 인만진;김민홍;정 진
  16. J. Microbiol. Biotechnol. v.1 Performance of column type bioreactor packed with immobilized cyclodextrin glucanotransferase for cyclodextrin production Lee, Y. H.;S. H. Lee;H. D. Shin
  17. Diaion™ Manual of ion exchange resins and synthetic adsorbent(2nd ed.) Mitsubishi Kasei Corporation
  18. US Patent 5215905 Immobilization of fructosyltransferase on a basic, porous anion-exchange resin Kim, M. H.;S. S. Choi;M. J. In;I. S.Choi;M. S. Han;B. S. Lim
  19. Biotechnol. Bioeng. v.22 Kinetic behaviour of immobilized penicillin acylase Carleysmith, S. W.;P. Dunnill;M. D. Lilly
  20. J. Jpn. Soc. Starch Sci. v.29 Purification and some properties of cyclodextrin glucanotransferase from Bacillus stearothermophilus TC-60 Kitahata, S.;S. Okada
  21. 한국산업미생물학회지 v.20 Bacillus sp. E1이 생산하는 cyclodextrin glucanotransferase의 정제 및 특성 박처넛ㄱ;우의전;국승욱;서병철;박관화;임 훈
  22. J. Coll. Interface Sci. v.121 Conformational states of enzymes bound to surfaces Sandwick, R. K.;K. J. Schray