Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Streptomyces lividans에서 secE 유전자의 클로닝과 염기서열 결정

  • 김순옥 (명지대학교 자연과학연구소 생명과학과) ;
  • 서주원 (명지대학교 자연과학연구소 생명과학과 Tel. 82-335-30-6190 E-mail : jwsuh＠bioserver.myongji.ac.kr)
  • Published : 1997.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The secE gene of Streptomyces lividans TK24 was cloned by the polymerase chain reaction method with synthetic oligonucleo- tide primers designed on the basis of the nucleotide sequences of Streptomyces coelicolor secE-nusG-rplK operon. The deduced amino acid sequences of the SecE were highly homologous to those of other known SecE protein, that is 36.8%, 30.4%, 80.0%, and 80.9%, similarity to E. coli, Bacillus subtilis, Streptomyces griseus, Streptomyces virginiae SecE, respectively and exactly same with Streptomyces coelicolor SecE. It means that in spite of evolutionary differences, the genes for protein translocation machinery are highly conserved in eubacteria. The gene organization of secE-nusG-rplK is also similar to that of E. coli, B. subtilis, and streptomycetes.

Keywords

References

  1. Molecular Microbiol. v.4 no.2 Isolation of a sec Y homologue from Bacillus subtilis: evidence for a common protein export pathway in eubacteria Suh, J. W.;S. A. Boylan;S. M. Thomas;K. M. Dolan;D. M. Oliver;C. W. Price
  2. J. Mol. Evol. v.29 Spectinomycin operon of Micrococcus luteus: Evolutionary implications of organization and novel codon usage Ohama, T.;A. Muto;S. Osawa
  3. 한산미지 v.23 no.6 Streptomyces coelicolor에서 secY 유전자의 클로닝과 염기서열 결정 김상숙;현창구;김영민;이주헌;정인권;김대명;서주원
  4. Streptomyces lividans에서 secY homolog의 클로닝과 분석 김순옥;서주원
  5. J. Bacteriol. v.150 Identification of a new gene(secA) and gene product involved in the secretion of envelope proteins in Escherichia coli Oliver, D. B.;J. Beckwith
  6. J. Bacteriol. v.154 Mutations in a new gene, secB, cause defective protein localization in Escherichia coli Kumamoto, C. A.;J. Beckwith
  7. J. Bacteriol. v.169 secD a new gene involved in protein export in Escherichia coli Gardel, S. C.;S. Benson;J. Hunt;S. Michaelis;J. Beckwith
  8. Genes Dev. v.3 The secE gene encodes an integral membrane protein required for protein export in Escherichia coli Schatz, P. J.;P. D. Riggs;A. Jacq;M. J. Fath;J. Beckwith
  9. EMBO J. v.13 no.9 The secD locus of E. coli codes for two membrane proteins required for protein export Gardel , C.;K. Johnson;A. Jacq;J. Beckwith
  10. EMBO J. v.13 Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature Nishiyama, K.;M. Honda;H. Tokuda
  11. Cell. v.32 A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins Ito, K.;M. Wittekind;M. Nomura;K. Shiba;T. Yura;A. Mjura;H. Nishimoto
  12. EMBO J. v.14 The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE Flower, A. M.;R. S. Osborne;T. J. Silhavy
  13. J. Biological. Chem. v.271 In vivo analyses of interactions between SecE and Sec Y, core components of the Escherichia coli protein translocation machinery Pohlschroder, M. C. Murphy;J. Beckwith
  14. J. Bacteriol. v.172 Sequence and transcriptional pattern of the essential Escherichia coli secE-nusG operon Downing, W. L.;S. L. Sullivan;M. E. Gottesman;P. P. Dennis
  15. Mol. Microbiol. v.10 Isolation and characterization of the secE homologue gene of Bacillus ubtilis Jeong, S. M.;H. Yoshikawa;H. Takahashi
  16. Mol. Gen. Genet. v.247 Cloning, nucleotide sequence, and transcriptional analysis of the nusG gene of Streptomyces coelicolor A3(2), which encodes a putative transcriptional antiterminator Puttikhunt, C.;T. Nihira;Y. Yamada
  17. Biochim. BIophys. Acta. v.1217 Organization and nucleotide sequence of the secE-nusG region of Streptomyces griseus Miyaka, K.;H. Onaka;S. Horinouchi;T. Beppu
  18. Genbank data Streptomyces virginiae VbrA gene for NusG like protein, SecE like protein and ribosomal protein, aspartate aminotransferase and adenosine deaminase Katayama, M.;Y. Sakai;S. Okamoto;F. Ihara;T. Nihira;Y. Yamada
  19. FEMS Micribiol. Lett. v.117 The Staphylococcus carnosus secE gene: cloning, nucleotide sequence, and functional characterization in Escherichia coli secE mutants strains Meens, J.;M. Klose;R. Freudl
  20. Genetic manipulation of Streptomyces, A laboratory munual Hopwood, D. A.;M. J. Bibb;K. F. Chater;T. Kieser;C. J. Bruton;H. M. Kieser;D. J. Lydiate;C. P. Smith;J. M. Ward;H. Schrempf
  21. Molecular cloning, A laboratory manual(2nd edition) Sambrook, J.;E. F. Fritsch;T. Maniatis