한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제25권2호
  • /
  • Pages.207-211
  • /
  • 1997
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Bacillus licheniformis NS70으로부터 내열성 Alkaline Protease 생산을 위한 배지최적화

  • 구자협 ((주)농심 기술개발연구소) ;
  • 최인재 ((주)농심 기술개발연구소) ;
  • 남희섭 ((주)농심 기술개발연구소) ;
  • 이형재 ((주)농심 기술개발연구소) ;
  • 신재의 ((주)농심 기술개발연구소) ;
  • 오태광 (생명공학연구소)
  • 발행 : 1997.04.01
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초록

Media optimization for the production of thermostable protease specifically hydrolyzing defatted soybean meal (DSM) from Bacillus licheniformis NS70 was performed by two methods, one-at-a-time method and response surface methodology (RSM). The best carbon source and nitrogen source for the protease production were lactose and DSM, respectively. The maximum protease production estimated by RSM was 606 U/L at 1.11% lactose and 0.43% DSM, the value of which was nearly consistent to the experimental value of 599 U/L. Yeast extract suppressed the protease production. The medium pH was slightly increased at the beginning stage of fermentation, and it tended to decrease after 8 hours. The optimal pH for the protease production was 7.2 in the batch fermentation.

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