Bacillus licheniformis NS70으로부터 내열성 Alkaline Protease 생산을 위한 배지최적화

  • 구자협 ((주)농심 기술개발연구소) ;
  • 최인재 ((주)농심 기술개발연구소) ;
  • 남희섭 ((주)농심 기술개발연구소) ;
  • 이형재 ((주)농심 기술개발연구소) ;
  • 신재의 ((주)농심 기술개발연구소) ;
  • 오태광 (생명공학연구소)
  • 발행 : 1997.04.01

초록

Media optimization for the production of thermostable protease specifically hydrolyzing defatted soybean meal (DSM) from Bacillus licheniformis NS70 was performed by two methods, one-at-a-time method and response surface methodology (RSM). The best carbon source and nitrogen source for the protease production were lactose and DSM, respectively. The maximum protease production estimated by RSM was 606 U/L at 1.11% lactose and 0.43% DSM, the value of which was nearly consistent to the experimental value of 599 U/L. Yeast extract suppressed the protease production. The medium pH was slightly increased at the beginning stage of fermentation, and it tended to decrease after 8 hours. The optimal pH for the protease production was 7.2 in the batch fermentation.

키워드

참고문헌

  1. 酵素による食品開發-最近の話題 v.31 no.2 食品と開發
  2. Cereal Foods World v.40 no.10 New proteolytic enzymes for the production of savory ingredients Pommer, K.
  3. 調味料製造用 酵素製劑 v.11 no.6 フレ-バ-ザイムについて 月刊フ-ドケミカル
  4. 대한민국특허 96-5057 향미료의 제조방법 탕호닥;로버트 두스탄 우드;알프레드 우페이어
  5. J. Microbiol. Biotechnol. v.6 no.1 Purification and characterization of an alkaline protease from Bacillus licheniformis NS70 Kim, Y. O.;J. K. Lee;Y. S. Park;T. K. Oh
  6. J. Ferment. Bioeng. v.72 no.1 Medium optimization by an orthogonal array design for the growth of Methanosarcia barkeri Silveira, R. G.;T. Kakizono;S. Takemoto;N. Nishio;S. Nagai
  7. Indian J. Microbiol. v.33 no.1 Optimization of alkaline protease production by thermophilic Bacillus licheniformis S-40 Shalini Sen;T. Satyanarayana
  8. Enzyme Microb. Technol. v.14 Production and degradation of alkaline protease in batch cultures of Bacillus subtilis ATCC 14416 Chu, I. M.;C. Lee;T. S. Li
  9. Appl. Microbiol. Biotechnol. v.22 A continuous culture study of bioenergetic aspect of growth and production of exocellular protease in Bacillus licheniformis Frankena, J.;H. W. van Verseveld;A. H. Stouthamer
  10. J. Gen. Microbiol. v.73 Continuous culture studies on the biosynthsis of alkaline protease, neutral protease and amylase Bacillus subtills NRRL-B 3411 Heineken, F. G.;R. J. O'Conner
  11. J. Ferment. Technol. v.65 no.3 Production of alkaline protease in a low-cost medium by alkalophilic Bacillus sp. and properties of the enzyme Fujiwara, N.;K. Yamamoto
  12. Appl. Environ. Microbiol. v.51 no.2 Effects of cysteine on growth, protease production, and catalase activity of Pseudomonas fluorescens Himelbloom, B. H.;H. M. Hassen
  13. Trends Food Sci. Technol. v.5 Systematic experimental designs for product formula optimization Arteaga, G. E.;E. Li-Chan;M. C. Vazquez-Arteaga;S. Nagai
  14. J. Food Sci. v.57 no.1 Edible wheat gluten films: influence of the main process variables on film properties using response surface methodology Gontard, N.;S. Guilbert;J. Cuq
  15. JAOCS v.72 no.6 Four-factor response surface optimization of the enzymatic modification of triolein to structured lipids Shieh, C. J.;C. C. Akoh;P. E. Koehler
  16. J. Gen. Physiol. v.30 Grystalline soybean trypsin inhibitor Kunitz, M.
  17. Agric. Biol. Chem. v.55 no.12 Production of thermostable alkaline protease by alkalophilic thermoactinomyces sp. HS682 Tsuchiya, K.;Sakashita, Y.;Nakamura, Y.;Kimura, T.
  18. Biotechnol. Bioeng. v.37 A parametric study of protease production in batch and fed-batch cultures of Bacillus firmus Moon, S. H.;Parulekar, S. J.