Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Penicillium sp.-L4의 균성장 및 효소작용을 억제하는 $\beta$-glucosidase inhibitor의 분리 및 특성

  • 김무성 ((주)태평양 기술연구원 기반기술연구소) ;
  • 하성윤 ((주)태평양 기술연구원 기반기술연구소) ;
  • 전기붕 ((주)태평양 기술연구원 기반기술연구소) ;
  • 임달택 ((주)태평양 기술연구원 기반기술연구소) ;
  • 박병화 ((주)태평양 기술연구원 기반기술연구소) ;
  • 이보섭 ((주)태평양 기술연구원 기반기술연구소) ;
  • 이상린 ((주)태평양 기술연구원 기반기술연구소) ;
  • 최영길 (한양대학교 생물학과)
  • Published : 1997.04.01
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Abstract

A producer of inhibitor against ${\beta}-glucosidase$ of Penicillium sp.-L4 was screened from Actinomycetes, and the isolated strain was identified as Streptomyces sp. The inhibitor produced was very stable against heat, acidic and alkaline conditions, proteolytic and amylolytic enzymes. The inhibotor was purified from culture broth through activated carbon treatment, ultrafiltration, anion and cation exchange, activated carbon columm, acetone precipitation and preparative HPLC. It showed inhibitory activities against a variety of dissacharide hydrolyzing enzymes produced by P.sp.-L4, and the mode of inhibition was competitive. Its structure and molecular formular was elucidated by IR, $^1H\;and\;^{13}C$ NMR and FAB/Mass spectrometry, which was identified as 1-deoxynojirimycin (dNM). dNM showed inhibitory effects on the cell growth and hydrolytic enzyme action of P.sp.-L4 on agar plate and infected lemon peel.

Keywords

References

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