인간 신경아세포종 세포 배양을 통한 뇌 신경세포 생육 촉진인자의 생산

  • 홍종수 (강원대학교 식품생명공학부) ;
  • 우광회 (한림대학교 의과대학 의학부) ;
  • .박경유 (한림대학교 의과대학 의학부) ;
  • 이현용 (강원대학교 식품생명공학부)
  • 발행 : 1997.02.01

초록

In cultivating human neuroblastoma cells maximum number of neurites per cell and length of the neurite were estimated as 5.5 and 2.2 (nm), respectively It was found that there was correlation between growth and differentiation of nerve cells. Maximum specific BDNF production rate was also calculated as 2.5$\times $10$^{-5}$(ng/cell/day) at 7$\times $ 10$^{5}$ (viable cells/ml) of maximum cell density, corresponding to 100 (ng/mL) of BDNF. The secretion of BDNF was occurred most in the later peroids of the cultivation, yielding 75 (ng/mL) of BDNF. The production of rate of BDNF was elongated in adding 1 ($\mu $g/mL) of BDNF as well as 40% increase of the length of the BDNF. It proves that BDNF can be used as one of biopharmaceuticals to treat age-related diseases such as Alzheimer's disease and Prakinson's disease. It can also provide the information of scaling-up mammalian cell cuture system to economically produce BDNF.

키워드

참고문헌

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