Lactobacillus plantarum을 용균시키는 Bacteriophoge SC921의 분리 및 특성

  • Yoon, Sung-Sik (Department of Biological Resources and Technology, Yonsei University) ;
  • Shin, Young-Jae (OTTOGI Corporation Research Center) ;
  • Choi, Hak-Jong (Department of Biological Resources and Technology, Yonsei University) ;
  • Her, Song (Department of Food and Biotechnology, Yonsei university) ;
  • Oh, Doo-Hwan (Department of Food and Biotechnology, Yonsei university)
  • 윤성식 (연세대학교 생물자원공학과) ;
  • 신영재 ((주)오뚜기 중앙연구소) ;
  • 최학종 (연세대학교 생물자원공학과) ;
  • 허송 (연세대학교 식품.생물공학과) ;
  • 오두환 (연세대학교 식품.생물공학과)
  • Published : 1997.02.01

Abstract

Among the lactic flora responsible for the development of acidity and characteristic flavor of Kimchi which is a traditional fermented Chiness cabbage. Homofermentative Lactobacillus plantarum is rod-shaped and to be known to ewert major role during later fermentation period. Once this strain establishes main flaora in the Kimchi fermentation process, it gives rise to excess acid production to reduce the taste and quality of Kimchi during storage. As a primary work to increase the keeping quality using virulent Lactobaillus plantarum bacteriophages, it was isolated sucessfully from collected Kimchi samples and their characteristics were studied. The new isolated phage, named SC 921, adsorbed to its host without Ca$^{2+}$, and nearly eliminated at 60$\circ $C of heat treatment for 5 min. This phages were atable at pH 4~ 10 but inactivated below pH 3.0 or pH 11.0 above. The latent period, rise period, and burst size of this phage was 100 min, 120 min, 31$\pm $2pfu/ml, respectively. Electron micrograph showed the phages particles were unusually oval feature of head (dia 80~ 120 nm) without contractile tail.

Keywords

References

  1. 김치의 과학 심포지움 논문집 김치의 과학 한국식품과학회
  2. 한국식품과학회지 v.22 김치에서 젖산균의 분리 및 이들 세균들의 배추즙액 발효 심선택;경규항;유양자
  3. 한국식품과학회지 v.16 김치발효에 대한 온도 및 소금 농도의 영향 민태익;권태완
  4. 인하대학교 기초과학연구소 논문집 v.11 각 온도에서 김치 발효중 미생물의 천이 과정 박현근;임종락;한홍의
  5. Bergey's Manual of Determinative Bacteriology(8th ed.) Buchanan, R. E.;Gibbons, N. E.
  6. J. Appl. Bacteriol. v.13 A medium for the cultivation of Lactobacilli De Man, J. C.;Rogosa, M.;Sharpe, M. E.
  7. Appl. Environ. Microbiol. v.35 Effect of lactose concentration on the efficiency of plating of bacterioiphage on Streptococcus cremors Terzaghi, E.;Terzaghi, B. E.
  8. Microbiology v.140 Characterization of Φ 393-A2, a baceriophage that infects Lactobacillus casei Herrero, M.;Reyes-Gavilan, C. G. de los;Caso, J. L.;Suarez, J.E.
  9. Appl. Environ. Microbiol. v.54 Characterization of Lactobacillus bulgaricus bacteriophage ch2 Chow, J. J.;Batt, C. A.;Sinskey, A.J.
  10. J. Appl. Bacteriol. v.54 Isolation and characterization of a Lactobacillus plantarum bacteriophage isolated from a meat starter culture Trevors, K. E.;Holley, R. A.;Kempton, A. G.
  11. Advances in Virus Research Guidelines for bacteriophage characterization Ackerman, H. W.;Audurler, A.;Berthiaume, L.;Jones, L A.;Lauffer(ed.)
  12. Appl. Microbiol. Biotechnol. v.39 Characterization of a virulent Lactobacillus sake phage PWH2 Leuschner, R. G. K.;Arendt, E. K.;Hammes, W. P.
  13. The genetics of bateria and their viruses (2nd ed.) Hayes, W.
  14. N. Z. J. Dairy Sci. Technol. v.15 A comparison of prolate and small isometric headed lactic streptococcal bacteriophages Heap, H. A.;Jarvis, A. W.