방선균의 xylB 변이주에 의한 포도당 이성화효소의 생산

  • 주길재 (경북대학교 농과대학 농화학과) ;
  • 이인구 (경북대학교 농과대학 농화학과)
  • 발행 : 1997.02.01

초록

Streptomyces chibaensis J-59 did not grow in the culture medium containing only xylose or xylan as a carbon source, because it was defective in xylulokinase production; xylB mutant. S. chibaensis J-59 was able to produce xylanase and $\beta $-xylosidase as well as glucose isomerase. The glucose isomerase in S. chilbaensis J-59 was induced in the medium containing xylan or xylose which could be utilized as an inducer but not sa carbon and energy sources. So we tried to produce glucose isomerase whthout consumption of xylose or xylan as an inducer by using xylB mutant S. chilbaensis J-59. The optimum condition for the production of the glucose isomerase was attained in a culture medium composed of 1% xylan, 0.15% glucose, 1.5% corn steep liquor, 0.1% MaSO$_{4}$ $\CDOT $7H$_{2}$O, and 0.012% CoCL$_{2}$ $\CDOT $ 6H$_{2}$O(pH 7.0). The production of the enzyme reached to a maximum level when the bacteria were cultured for 42 h at 30$\circ $C. The enzyme production in a jar fermentor was increased twice as much as that in a flask culture.

키워드

참고문헌

  1. J. Bacteriol. v.139 Uptake and catabolism of D-xylose in Salmonella typhimurium LT2 Shamanna, D. K.;K. E. Sanderson
  2. Mol. Gen. Genet. v.208 Cloning of the glucose isomerase (D- xylose isomerase) and xylulose kinase gene of Streptomyces violaceoniger Marcel, T.;D. Drocourt;G. Tiraby
  3. U.S. patent 4,532,208 Constitutive glucose isomerase producer Hafner, E. W.
  4. Appl. Microbiol. v.30 D-Glucose isomerase: Constitutive and catabolite repression-resistant mutant of Streptomyces phaeochromogenus Sanchez, S.;C. M. Quinto
  5. 한국산업미생물학회지 v.25 no.1 내열성 포도당 이성화효소를 생산하는 Streptomyces sp. J-59의 분리 및 동정 주길재;권기석;이인구
  6. J. Ferment. Technol. v.54 The measurement of celluar protein content as a method for determining mold concentration Schmidell, W.;M. V. Fernandes
  7. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantites of protein dye binding Bradford, M. M.
  8. J. Biol. Chem. v.192 A new spectrophotometric method for the detection and determination of keto sugars and trioses Dische, Z.;E. Borenfreund
  9. Methods Enzymol. v.9 D-xyluokinase Simpson, F. J.
  10. Biotechnol. lett. v.2 Simultaneous production of cellulase complex and glucose isomerase by S. flavogriseus Kluepfel, D.;L. Biron;M. Ishaque
  11. Anal. Chem. v.31 Use of dinitrosalicyclic acid reagent for determination of reducing sugar Miller, G. L.
  12. Int. J. Syst. Bacteriol. v.16 Methods for characterization of Streptomyces species Shirling, E. B.;D. Gottlieb
  13. Agric. Biol. Chem. v.29 Enzymatic conversion of D-glucose to D-fructose. Part II. Properties of the enzyme from Streptomyces phaeochromogenes Tsumura, N.;M. Hagi;T. Sato
  14. 한국산업미생물학회지 v.17 Streptomyces sp. GI32 방선균의 glucose isomerase 생산과 효소특성 서형주;김진만;이태경;양한철
  15. Agric. Biol. Chem. v.28 Studies glucose isomerase of bacteria. Part II. The glucose isomerizing activity of Escherichia intermedia Natake, M.;S. Yoshimura
  16. J. Paper Trade v.122 Chlorite holocellulose, its fractionation and bearing on summative wood analysis and on studies on the hemicellulose Wise, L. E.;M. Murphy;A. A. D'Aieco
  17. 木材學會誌 v.27 熱葉産 廣葉樹材 キシラン に關する 硏究 李種潤;趙南奭;石律丁;中野準三
  18. 한국 펄프·종이 공학회 v.18 참나무류의 hemicellulose 중 xylan 이종윤;엄태진
  19. Agric. Biol. Chem. v.29 Enzymatic conversion of the D-glucose to D-fructose. Part VI. Properties of the enzyme from Streptomyces phaechromogenus Tsumura, N.;T. Sato
  20. Agric. Biol. Chem. v.30 Studies on sugar isomerization enzyme. Production and utilization of glucose isomerase from Streptomyces spp. Takasaki, Y.
  21. Appl. Environ. Microbiol. v.32 Studies on glucose isomerase from S. grisefuscus S41 Chou, C. C.;M. R. Ladisch;G. T. Tsao
  22. Agric. Biol. Chem. v.45 Subunit structure of glucose isomerase from a Streptomyces sp. Kasumi, T.;K. Hayashi;N. Tsumura
  23. 한국산업미생물학회지 v.19 포당당 이성화 효소 생산성 신균주 Streptomyces luceogriseus의 분리 및 발효특성 홍승서;백진기;이현수;국승욱;박관화
  24. J. Ferment. Technol. v.63 Biotransformation of D-xylose to D-glucose by immobilized enzyme prepared from Streptomyces flavorinensis Ohmomo, S.;T. Shikawk,;Y. Kurosaki;T. Hashizume;Y. Tozawa;K. Ueda
  25. Appl. Microbiol. Biotechnol. v.43 Isolation and characterization of a D-glucose/xylose isomerase from a new thermophilic strain Streptomyces sp. PLC Inyang, C. U.;U. Gebbart;S. K. C. Obi;H. Bisswanger