Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Halomonas sp. ES-10균주가 생산하는 효소세제용 알칼리성 Protease

  • 김찬조 (충남대학교 식품공학과) ;
  • 이재숙 (충남대학교 식품공학과) ;
  • 최성현 ;
  • 오만진 (충남대학교 식품공학과)
  • Published : 1997.02.01
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Abstract

To utilize the alkaline protease produced by Halomonas sp. ES-10 as an enzyme detergent, the crude enzyme was obtained by methanol precipitation and lyophilization. And it was processed to coated enzyme.The best mixing ratio of components such as coated enzyme, builders, actives, fillers and adjuvants on detergency was examined, and temperature and pH influencing detergency were also tested. Detergency test 0.15% detergent solution was carried out on EMPA test cloth #116 with shaking(90 rpm) for 10 min after 30 min of pretreatment. The detergent which contained coated-enzyme 1%, Zeolite 4A 20%, Tween 80 1. 5%, sodium borate 30%, sodium meta silicate 7.5% and water 40% showed about 90% of washing efficiency at 40$\circ $C and pH 10.0.

Keywords

References

  1. Microbiology of Extreme Environments Edward, C.
  2. J. Bacteriol. v.119 Halophilic nuclease from a moderately halophilic Micrococcus varians Kamekura, M.;H, Onish
  3. Agric. Biol. Chem. v.34 Enzymatic properties of purified alkaline proteinase from Aspergillus sojae Hayashi, K.;M. Terada;K. Mogi
  4. Agric. Biol. Chem. v.36 Enzymatic propeties of alkaline proteinase from Aspergillus candidus Ohara, T.;S. Nasuno
  5. J. Bacteriol. v.171 Isolation, purification and properties of Penicilium charlesii alkaline protease Abbas, C. A.;S. Groves;J. E. Gander
  6. Agric. Biol. Chem. v.35 Production of alkaline enzymes by alkalophilic microorganisms part I. Alkaline protease produced by Bacillus No. 221 Horikoshi, K.
  7. Agric. Biol. Chem. v.36 Purification and properties of proteases from a marine-psychrophilic bacterium Kato, N.;T. Nagasawa;S. Adachi;Y. Tani;K. Ogata
  8. Agric. Biol. Chem. v.38 Cleavage site specificities of silkworm alkaline proteases Sasaki, T.;M. Morishita;M. Ise
  9. Agric. Biol. Chem. v.49 Cleavage site specities of silkworm alkaline proteases Sasaki, T.;M. Morishita;M. Ise
  10. J. Bacteriol. v.172 Cloning of the Pseudomonas aeruginosa alkaline protease gene and secretion of the protease into the medium by Escherichia coli Gazzo, J.;M. Murgier;A. Filloux;A. Lazdunski
  11. 한국농화학회지 v.35 Halomonas sp. ES 10에 의한 alkaline protease의 생산 김찬조;김교창;오만진;최성현
  12. 한국농화학회지 v.35 Halomonas sp. ES 10이 생산하는 alkaline proteased의 특성 김찬조;오만진;최성현
  13. J. Gen. Microbiol. v.55 Proteolytic enzyme from extremely halophilic bacteria Norberg, P.;B. V. Hofsten
  14. Patent specification 1362356 Courier Press;Leamington Spa
  15. 한국농화학회지 v.31 맥아의 α-amylase isozyme에 미치는 red light의 영향 김진구;신승렬;김광수;손태화
  16. JAOCS v.62 Lipid-lipase interactions. 2. A new method for the assay of lipase activity Linfield, W. M.;S. Serota;L. Sivieri
  17. JAOCS v.67 The effect of builders on the activity of protease enzymes Sato, M.;K. Yoshikawa;M. Minagawa
  18. 한국의류학회지 v.3 세제의 종류 및 세척 온도에 따른 각종섬유질의 세척성 조성교