Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Aspergillus niger SFN-416으로부터 생산한 $\beta$-Glucosidase의 정제 및 특성

  • 성찬기 (중앙대학교 화학과) ;
  • 이상원 (진주산업대학교 미생물공학과) ;
  • 박석규 (순천대학교 식품영양학과) ;
  • 박정로 (순천대학교 식품영양학과) ;
  • 문일식 (순천대학교 화학공학과)
  • Published : 1997.02.01
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Abstract

$\beta $-Glucosidase (EC 3.2.1.21) was purified from Aspergillus niger SFN-416 by a sequential process of ammonium sulfate precipitation, Sepadex G-100 and DEAE-Sephacel column chromatography. Molecular weight of the enzyme was 46, 000 daltons. The K$_{m}$ and V$_{max}$ values for PNPG were 0.67 mM and 25 moles/ml $\cdot $min., respectively. The optimum pH and temperature of the enzyme activity were 3.5 and 58$\circ $C, respectively. The enzyme activity was decreased by addition of metal ions, and increased by addition of metanol, ethanol, isopropanol and 1-butanol at a concentration of 10% (v/v). Stability of the enzyme was increased by addition of isopropanol and 1-butanol at a concentration of 10% (v/v).

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