Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Transcriptional Regulation of the Drosophila Proliferating Cell Nuclear Antigen Gene and raf Proto-oncogene by Ursolic Acid in Drosophila Cultured Kc Cells

  • Park, Thae-Yeong (Department of Bionlogy, College of Natural Sciences, Pusan National University) ;
  • Rhee, Sang-Hoon (Department of Bionlogy, College of Natural Sciences, Pusan National University) ;
  • Kim, Han-Do (Department of Bionlogy, College of Natural Sciences, Pusan National University) ;
  • Kim, Chong-Rak (Department of Bionlogy, College of Natural Sciences, Inje National University) ;
  • Kang, Ho-Sung (Department of Bionlogy, College of Natural Sciences, Pusan National University, Pusan 609-735,Korea, Department of Bionlogy, College of Natural Sciences, Pusan National University, Pusan 609-735,Korea, Department of Bionlogy, College of Natural Sciences, inje University, Kimhae 621-749,Korea, Department of Bionlogy, College of Natural Sciences, Pusan National University) ;
  • Yoo, Mi-Ae (Department of Bionlogy, College of Natural Sciences, Pusan National University)
  • Published : 1997.03.01
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Abstract

Promoter of the Drosophila proliferating cell nuclear antigen (PCNA) gene contains DRE (Drosophila DNA replication-related element) required for the high level expression of replication-related genes. Recently, we found that promoter region of the D-raf (a Drosophila homolog of the human c-raf-1) contains two sequences homologous to the DRE and demonstrated the DRE/DREF (DRE-binding factor) involvement in regulation of the D-raf gene. In this study, using ursolic acid (UA), a pentacyclic triterpene acid reported to possess antitumor activities, we examined effects of UA on proliferation of the Drosophila cultured Kc cells and on expression of the PCNA and D-raf genes. UA showed an inhibitory effect on proliferation of the Kc cells in a concentration-dependent manner in DNA content assays and [3H]thymidine incorporation assays. The IC50 value of anti-proliferative effects of UA in DNA content assays was about 7.5uM. UA showed inhibitory effects on expression of the PCNA as well as on that of the D-raf, which were examined with the reporter plasmic p5'-168DPCNACAT or p5'-878DrafCAT, respectively. The results obtained in the present study suggest that expression of the PCNA and D-raf genes is coordinately regulated in at least UA-treated Kc cells and that down-regulation of expression of the PCNA and D-raf genes might be related with the antitumor activities of UA.

Keywords

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