Biotechnology and Bioprocess Engineering:BBE
- 제2권2호
- /
- Pages.113-116
- /
- 1997
- /
- 1226-8372(pISSN)
- /
- 1976-3816(eISSN)
Separation and Purification of Useful Proteins Using Hydrogel Ultratiltration
-
Park, Chang-Ho
(Department of Chemical Engineering, Kyung Hee University) ;
- Son, Chang-Kyu (Department of Chemical Engineering, Kyung Hee University) ;
-
Park, Jong-Hwa
(Department of Chemical Engineering, Getetic Engineering, Kyung Hee University) ;
-
Chung, In-Sik
(Department of Chemical Engineering, Getetic Engineering, Kyung Hee University)
- 발행 : 1997.12.01
초록
The hydrogel process is a different form of ultrafiltration and has been used to separate biological molecules. In this study, the gel pore size was predicted by pulse NMR technique and neural network using a database obtained from gel filtration chromatography and diffusion experiment. Recombinant alkaline phosphatase expressed in insect cells was concentratred 1.5 times by hydrogel ultrafiltration by swelling at 2
키워드