Biotechnology and Bioprocess Engineering:BBE
- Volume 2 Issue 2
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- Pages.86-89
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- 1997
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- 1226-8372(pISSN)
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- 1976-3816(eISSN)
Expression of Recombinant Epidermal Growth Factor in E. coli
- Chang Shin Yoon (Department of Biochemistry and Molecular Biology) ;
- Eun (Department of Chemical Engineering, Hanyang University Department of Biochemistry and Molecular Biology Department of Biochemistry and Molecular Biology)
- Published : 1997.12.01
Abstract
Epidermal growth factor(EGF) known as a urgastrone is a powerful mitogen with a wide variety of possibilities for medical usages. A mature EGF coding region was isolated from human prepro-EGF sequence by a conventional PCR and cloned into pQE vector in which the gene product was supposed to be expressed with 6