생명과학회지 (Journal of Life Science)

  • 제7권4호
  • /
  • Pages.392-401
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  • 1997
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

폴리오바이러스의 분자생물학

Molecular Miology of the Poliovirus

  • 최원상 (동국대학교 자연과학부 생화학전공)
  • 발행 : 1997.12.01
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초록

폴리오바이러스는 바이러스들 중에서도 특히 커기가 작은 바이러스로서 피막(coat)을 둘러싸는 막(envelop) 이 없다. 폴리오바이러스는 (+) 가닥의 단일 RNA 게놈을 갖는데 이는 한 개의 해독판 (open reading frame)을 이용하여 다단백전구체를 만든 후 바이러스 자체의 단백질분해효소에 의해 스스로 잘라져서 궁극적으로느 특이한 기능을 갖는 여러개의 단백질이 된다. P1 다단백질전구체로부터 만들어지는 단백질들은 바이러스의 피막을 구성하는 성분이다. 단백질분해효소인 2A에 의한 최초의 절단은 구조단백질 P1 전구체와 구조단백질이 아닌 P2-P3간을 분리시켜준다. 단백질분해효소 2A는 진핵세포 판독개시인자(translation initiation factor) 4F의 한 subunit인 숙주단백질 p220의 절단에 간접으로 참여한다. 이 단백질의 절단은 캡(cap)에 의존하는 숙주세포의 대부분의 판독을 차단하게 되며 이는 판독에 사용되는 숙주세포의 모든 기구들을 캡에 의존하지 않는 폴리오바이러스 NA 특유의 판독을 위해 전적으로 사용할 수 있게 해준다. 2B, 2C, 2BC 단백질의 기능에 대해서는 많이 알려져 있지 않다. 2B, 2C, 2BC와 3CD 단백질들은 바이러스로 인해 만들어지는 소낭(vesicle)의 복제복합체에 함유되어 있으므로 바이러스의 RNA 복제시 중요한 역할을 함을 암시해준다. 새로이 만들어진 모든 바이러스 RNA는 VPg와 공유결합으로 연결되어 있다. VPg는 3AB로부터 만들어진 아미노산 22개 짜리의 폴리펩타이드이다. 3C와 3CD는 단백질분해소로 다단백질 전구체의 대부분의 절단부위를 잘라준다. 3C단백질은 숙주의 전사인자를 불활성화 시킴으로써 RNA polymer II와 III에 의한 전사를 저해한다. 3D는 RNA의존선RNA 중합효소이다. 폴리오바이러스는 (+)가닥 RNA 바이러스의 일반적인 복제양식을 따른다. 즉 (+) 가닥 RNA는 이와 상보적인 (-)가닥 RNA로 전사되고 이는 다시 (+)가닥 RNA의 합성을 위한 주형으로 사용된다. 폴리오바이러스의 RNA 합성은 세포내막에서 일어나는 데 RNA 복제에 요구되는 주형 RNA와 이때 필요한 단백질들이 어떤 방법으로 세포내막에서 모일 수 있는지는 아직 밝혀진 것이 적다. 바이러스입자의 형성은 세포막의 RNA 복제가 들어가는 데 피막단백질이 (+)가닥 RNA을 인식하는 표지 즉 packaging singal에 대해서는 거의 알려져 있지 않다. 폴리오바이러스 감염 후 첫 바이러스입자가 만들어지기 까는 약 6시간이 소요된다.

The poliovirus is a small, and non-enveloped virus. The RNA genome of poliovirus is continuous, linear, and has a single open reading frame. This polyprotein precursor is cleaved proteolytically to yield mature products. Most of the cleavages occur by viral protease. The mature proteins derived from the P1 polyprotein precursor are the structural components of the viral capsid. The initial cleavage by 2A protease is indirectly involved in the cleavage of a cellular protein p220, a subunit of the eukaryotic translation initiation factor 4F. This cleavage leads to the shut-off of cap-dependent host cell translation, and allows poliovirus to utilize the host cell machinery exclusively for translation its own RNA, which is initiated by internal ribosome entry via a cap-independent mechanism. The functional role of the 2B, 2C and 2BC proteins are not much known. 2B, 2C, 2BC and 3CD proteins are involved in the replication complex of virus induced vesicles. All newly synthesized viral RNAs are linked with VPg. VPg is a 22 amino acid polypeptide which is derived from 3AB. The 3C and 3CD are protease and process most of the cleavage sites of the polyprotein precursor. The 3C protein is also involved in inhibition of RNA polymerase II and III mediated transcription by converting host transcription factor to an inactive form. The 3D is the RNA dependent RNA polymerase. It is known that poliovirus replication follows the general pattern of positive strand RNA virus. Plus strand RNA is transcribed into complementary minus strand RNA that, in turn, is transcribed for the synthesis of plus strand RNA is transcribed into complementary minus strand RNA that, in turn, is transcribed for the synthesis of plus strand RNA strands. Poliovirus RNA synthesis occurs in a membranous environment but how the template RNA and proteins required for RNA replication assemble in the membrane is not much known. The RNA requirements for the encapsidation of the poliovirus genome (packaging signal) are totally unknown. The poliovirus infection cycle lasts approximately 6 hours.

키워드

참고문헌

  1. Biochem. Biophys. Rec. Comm. v.206 Induction of membrane proliferation by poliovirus protein 2C and 2BC Aldabe, R.;Carrasco, R.
  2. J. Virol. v.71 Poliovirus protein 2BC incerases cytosolic free calcium concentrations Aldabe, R.;Irurzun, A.;Carrasco, L.
  3. Cell v.15 An enzymatic activity in uninfected cells that cleaves the linkage between poliovirus RNA an dthe 5' terminal protein Ambros, V.;Pettersson, R. F.;Baltimore, D.
  4. EMBO J. v.12 Poliovirus RNA synthesis utilizes an RNP complex formed around the 5' and of vital RNA Andino, R.;rieckhof, G. E.;Achacoso, P. L.;Baltimore, D.
  5. Cell v.66 A functional ribonucleoprotein complex foems around the 5' end of poliovirus RNA Andino, R.;Rieckhof, G. E.;Baltimore, D.;
  6. Proc. Natl. Acad. Sci. v.3 Purigicatio of a terminal uridyllyltransterase that acts as host factor in the in vitro poliovirus replicase reaction Andrews, N. G.;Baltimore, D.
  7. J. Virol. v.66 Myristylation of poliovirus capsid precursor P1 is requires for assembly of suviral particles Ansardi, D. C.;Porter, D. C.;Morrow, C. D.
  8. Biochim. Biophys. Acta. v.76 Altered location of perotein synthesis in the cell agter poloiovirus ingections Baltimore, D.;Eggers, H.;Tamm, I.
  9. J. Virol. v.6 Cap-independent translatio by the 5' untranslated region of Theiler's murine encephalomuelitis virus Bandopadhyay, P. K.;Wang, C.;Lipron, H.
  10. J. Virol. v.69 An internal tibosomal rntey mechanism promotes translation of murine leukemia virus Gag polyprotein percursors Berlioz, C.;darlix, J. L.
  11. J. Virol. v.60 Gnetic complementation among poliovirus mutants derived from an infectious cDNA clone Bernstein, H. D.;Sarnow, P.;Baltimore, D.
  12. J. Virol. v.66 Structural and functional characterization of the poliovirus replication complex Bienz, K.;Egger, D.;Pfister, T.;Troxler, M.
  13. EMBO J. v.13 Sequences within the poliovirus internal ribosome entry degment control viral RNA synthesis Borman, A. M;;Deliat, F. G.;Kean, K. M.
  14. J. Gen. Virol. v.74 The involvement of a splicesome component in internal of human rhinovirus RNA translation Borman, A. M.;Howell, T.;Patton, J. G.;Jackson, R. J.
  15. J. Gen. Virol. v.21 The formation of poliovirus particles in association with the RNA replication complex Caliguiri, L. A.;Compans, R. W.
  16. Virology v.46 Proteins associated with the poloiovirus RNA replication complex Caliguiri, L. A.;Mosser, A. G.
  17. Virology v.202 Membrane rearrangement and vesicle induction by recombinant poliovirus 2C and 2BC in human cells Cho, M. W.;Teterina, N.;Eger, D.;Bienz, K.;Ehrenfeld, E.
  18. J. Virol. v.65 Expression of human immunodeficiency virus type 1(HIV-1) gag, pol and env proteins from chimeric HIV-1 poliovitus minireplicons Choi, W. S.;Pal-Ghosh, R.;Morrow, C. D.
  19. EMBO J. v.10 Polivirus proteinase 3C converts an active form of transcription factor Ⅲc to an inactive form: a mechcanism foe inhibition of host cell polymerase Ⅲ transceiption by poliovirus Clark, M. E.;Hammerle, T.;Wimmer, E.;Dasgupta, A.
  20. Mol. Cel. Biol. v.13 Direct cleavage of human TATA-binding protein by poliovirus protease 3C in vivo an din vitro Clark, M. E.;Liberman, P. M.;Berk, A. J.;Dasgupta, A.
  21. Prog. Med.Virol. v.20 Defective intergering particles of poliovirus Cole, C. N.
  22. Proc. Natl. Acad. Sci. v.80 Genome-linked protein VPg of poliovirus is present as free VPg and VPg-pUpU in poliovirus-infected cells Crawford, N. M.;Baltimore, D.
  23. Proc. Natl. Acad. Sci. v.76 Polio replicase : a soluble enzyme able to initiate copying of poliovirus RNA Dasgupta, A.;Baron, M. H.;Baltimore, D.
  24. J. Virol. v.70 Reversible dissociation of the poliovirus replicarion complex : functions and interactions of its components in viral RNA synthesis Egger, D.;Pasamontes, L.;Bolten, R.;Boyko, V.;Vienz, K.
  25. EMBO J. v.8 Structural factors that control conformational transitions and serotype speificity in type 3 poliovirus Filman, D. J.;Syed, B.;Chow, M.;Macadam, A. J.;Minor, P. D.;Hogle, J. M.
  26. Proc. Natl. Acad. Sci. v.74 Poliovirus-specific primer-dependent RNA polymerase able to copy poiy(A) Flanegan, J. B.;Baltimore, D.
  27. J. Virol. v.71 The sequence element of the internal ribosome entry site and a 25kilodalton cellular protein contribute to efficient internal intiation of translatio of hepatitis C virus RNA Fukshi, S.;Kurihara, C.;Ishiyama, N.;Hoshino, F. B.;Oya, A.;Katayama, K.
  28. Arch. Ges. Virusforsch v.40 Inhibition of polivirus RNA sunthesis in an in vitro system by antiserum by antiserum against 14S virus specific structures Ghendon, Y. Z.;Babushkina, L.;Blagoveshienskaya, O.;
  29. J. Virol. v.65 Role of viral membrane pilypeptide in strand specific initiation of poliovirus RNA synthesis Giachetti, C.;Semler, B. L.
  30. Virology v.193 Ide tification of the hepatitis A virus internal ribosome entry site: in vivo an din vitro analysis of bicistronic RNAs contatining the HAV 5' noncoding region Glass, M. J.;Jia, X.;Summers, D.
  31. J. Virol. v.65 Catalysis of poliovirus VPO maturation cleavage is not mediated by Serine 10 of VP2 Harber, J. J.;Bradley, J.;Anderson, C. W.;Wimmer, E.
  32. J. Biol. Chem. v.269 Interaction of poliovirus polypeptide 3Cpro with the 5' and 3' termini of the poliovirus genome. Identification of viral and cellular cofactors needed for efficient binding Harris, K. S.;Xiang, W.;A;exander, L.;Lane, W. S.;Paul, A. V.;Wimmer, E.
  33. Seminars in Virology v.1 Proteolytic processinsg in the replication of picornavituses Harris, K. S.;Hellen, C. U. T.;Wimmer, E.
  34. J. Receptor v.4 the androgen receptor in the resticular feminized(Tfm) mouse may be a product if internal translation initiation He, W. W.;Lindzey, J. K.;Prescott, J. L.;Kumar, M. V.;Tindall, D.
  35. J. Virol. v.61 Host factor-induced template modification during synthesis of poliovorus RNA in vitro Hey, T. D.;Richards, O. C.;Ehrenfeld, E.
  36. Science v.229 Three dimensional structure of poliovirus at 2.9A resolution Hogle, J. M.;Chow, M.;Filman, D. F.
  37. Adv. Virus Res. v.42 Biology of coxakie A viruses Hyypia, T.;Stanway,G.
  38. Trands Biochem. Sci. v.15 The novel mechanism of initiation of picronavirus RNA translation Jackson, R. J.;Howell, M. T.;Kaminski, A.
  39. J. Virol. v.67 Biochemical and genetic evisence for a pseudoknot structure at the 3' terminus of the poliovirus RNA genome and its role in viral RNA amplication Jacobson, S. J.;Konings, D. A. M.;Sarmow, P.
  40. J. Virol. v.62 A segment of the 5' nontranslated tegion of encephalomyocarsitis virus RNA directs internal entry of ribosomes durong in vitro translation Jang, S. K.;Krausslich;H. G. Nicklin, M. J. H.;Duke, G. M.;Palmenberg, A. C.;Wimmer, E.
  41. J. Virol. v.70 Replication of hepatits A viruses with chimeric 5' nontranslated regions Jia, X.-Y.;Tesar, M.;Summers, D. F.;Ehrenfeld, E.
  42. J. Virol. v.71 Virus-encoded RNA helicases Kadare, G.;Haenni, A.
  43. Nature (London) v.291 Primary structure, gene organization and polypeptide expression of poliovirua RNA Kitamura, N.;Semler, B. L.;rothberg, P. G.;Larsen, G. R.;Adler, C. J.;Dorner, A. J.;Enimi, E. A.;Hanecak, R.;Lee, J. J.;van der Werf, S.;Anderson, C. W.;Wimmer, M.
  44. The molocular biology of poliovirus Koch, F.;Koch, G.
  45. J. Virol. v.62 Purification and partial charaterization of poliovitus protease 2A bymeans of a functional assay Konig, H.;Rosenwirth, B.
  46. Microbiological reviews v.47 Comparison of initiayion of protein synthesis in procaryotes, eucaryotes and organelles Kozak, K.
  47. J. Virol. v.61 Poliovirua proteinase 2 A indduces cleavage of eukaryotic initiation factor 4F polypeptide p220 Krausslich, H. J.;Nicklin, M. J. H.;Toyoda, H.;Etchison, D.;Wimmer, E.
  48. J. Virol. v.64 Myristoylation of the poliovirus polyprotein is reqired for proteolytic processing of the capsid and for viral infectivity Krausslich, H.;Holscher, C.;Reuer, Q.;Harber, J.;Wimmer, E.
  49. Virology v.198 Distinct structural elements and internal entry of ribosomes in mRNA3 endcoded by infectious bronchitis virus Le, S. -Y.;Sonenberg, N.;Maizel Jr., J. V.
  50. J. Virol. v.66 Internal entry of ribosomes on a tricistronic mRNA encoded by infectious bronchitis virus Liu, D. X. M.;Inglis, S. C.
  51. J. Virol. v.65 Interaction of a cellular 57kilodalton protein with the internal translation initiation site of foot-and- mouth disease virus Luz, N.;Beck, E. J.
  52. Nature v.353 Internal initiation of translation mediated by the 5'-leader of a cellular mRNA MAcejak, D. G.;Sarnow, P.
  53. Cell v.56 Cellulat receptor for poliovirus : molecular cloning, nucleotide sequence and expression of a new mwmber for the immunoglobulin superfamily Mendelsohn, C. L.;Wimmer, E.;Racaniello, V. R.
  54. Virology v.199 Biochemical studies on poliovirus polypeptide 2C : evidence foe ATPase activity Mirzayan, C.;Wimmer, E.
  55. Science v.254 Cell-free, denovo synthesis of poliovirus Molla, A.;Paul, A. V.;Wimmer, E.
  56. Cell v.40 The host protein required for in vitro replication of poliovirus is a protein kinase that phosphorylates eukaryotic initiation factor-2 Morrow, C. D.;Gibbons, G. F.;Dasgupta, A.
  57. J. Virol. v.50 ATP is required for initiation of poliovirus RNA synthesis in vitro : demonstration of tryosine-phosphate linkage between in vitro-synthesized RNA and genome linked protein Morrow, C. D.;Hocko, J.;Navab, M.;Dasgupta, A.
  58. J. Virol. v.65 Myristylation is important at multiple stages in poliovirus assembly Moscufo, N.;Simons, J.;Chow, M.
  59. Genes Dev. v.6 Homeotic gene Antennapedia mRNA contains 5'-noncoding sequences that confer translational initiation by internal ribosome binding Oh, S. K.;Scott, M. P.;Sarmow, P.
  60. Proc. Natl. Acad. Sci. v.84 Capsid protein VP4 of poliovirus is N-myristoylated Paul, A. V.;Schultz, A.;Pincus, S. E.;Oroszolan, S.;Wimmer, E.
  61. Mol. Cell. Biol. v.8 Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' non-coding region Pelletier, J.;Kaplan, G.;Racaniello, V. R.;Sonenberg, N.
  62. Nature v.334 Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from polivirus RNA Pelletier, J.;Sonenberg, N.;
  63. Nucleic Acids Res. v.20 Towards identification of cis-acting elements involved in the replication of entrovirus and rhinovirus RNas : a proposal for the existence of tRNA-like terminal structures Pilipenko, E. V.;Maslova, S. V.;Sinyakov, A. N.;Agol. V. I.
  64. Virology v.206 Pestivirus translation initiation occurs by internal ribosome entry Poole, T. L.;Wang, C.;Popp, R. A.;Potgieter, L. N.;Siddique, A.;Collet, M. S.
  65. J. Virol. v.67 Encapsidation of genetically engineered poliovirus minireplicons which experss human immunodeficiency virus type 1 Gag and Pol proteins upon ingection Porter, D. C.;Ansardi, D. C.;Choi, W. S.;Morrow, E. D.
  66. Science v.214 Cloned poliovirus complementary DNA is infections in mammalian cells Racaniello, V. R.;Baltimore, D.
  67. J. Virol. v.64 Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg Reuer, Q.;Kuhn, R. J.;Wimmer, E.
  68. Virology v.185 Identification of the initiation codon of plum pox potyvirus genomic RNA Riechamn, J. L.;Lain, S.;Garcia, J. A.
  69. J. Biol. Chem. v.268 Poliovirus protein-2C has ATPase and GTPase activities Rodriguez, P. L.;Carrasco, L.
  70. Viorology v.44 Comparison of polysomal structures of uninfected and poliovirus-infected HeLa cells Roumiantzeff, M.;Maizel Jr., J. V.;Summers, D. F.
  71. J. Virol. v.63 Inhibition of rRNA synthesis by poliovirus : specific inactivation of transcription factos Rubinstein, S. J;Dasgupra, A.
  72. Virology Rueckert, ,R. R.;B. N. Fields(ed.)
  73. J. Viorl. v.71 Poliovirus infection and expression of the poliovirus protein 2B provoke the disassembly of the Golgi complex, the organelle target for the anti-poliovirus drug R0-090179 Sandoval, I. V.;Carrasco, L.
  74. Proc. Natl. Acad. Sci. v.83 A poliovirus temperature-sensitive RNA synthesis mutant licated in a non-coding region of the genome Sarnow, P.;Bernsteine, H. D.;Baltimore, D. A.
  75. J. Virol. v.70 Cellular origin and ul trastructure of membranes induced during poliovirus infection Schlegel, A.;Gidding Jr., T. H.;Lasindky, M. S.;Kirkgaard, K.
  76. J. Virol. v.71 Host range phenotype induced by mutations in the internal rebosomal entry site of poliovirus RNA Shiroki, K.;Ishil, T.;Aoli, T.;Otas, Y.;Yang, W-X.;Komatsu, A.;Ami,, Y.;Orita, M.;Abr, S.;Hashizume, S.;Nomoto, ,A
  77. J. Virol. v.60 Initiation of poliovirus plus-strand RNA sunthesis in a membrane complex of infected HeLa cells Takeda, N.;Kuhn, R. J.;Yang, C-F.;Takegami, T.;Wimmer, E.
  78. J. Virol. v.52 Association of poliovirus proteins with the endoplasmic reitculum Tershak, D. R.
  79. Cell v.45 A second virus encoded profease involved in protelytic process of poliovirus polyprotein Toyoda, H.;Nicklin, M. J. H.;Murray, M. G.;Anderson, C. W.;Dunn, J. J.;Studier, F. W.;Wimmer, E.
  80. J. Viol. v.66 Internal ribosome entru site within hepatitis C virus RNA Tsukiyama-Kohara, K. I.;Zuka, N.;Kohara, M.;Nomoto, A.
  81. J. Virol. v.35 Identification penfent RNA polypeptide p63 as a soluble RNA-dependent RNA polymerase Van Dyke, T. A.;Flanegan, J. B.
  82. Cell v.28 Genome-linked protein of viruses Wimmer, E.
  83. J. Cell. Sci. Supple v.7 Molecular events leasing to piconavirus genome replication Wimmer, E.;Kuhn, R. J.;Pincus, S.;Yang, C.-F.;Toyoda, H.;Nicklin, M. J. H.;Takeda, N.
  84. Pro. Natl. Acad. Sci. v.87 Eukaryotic initiation factor 3 is required for poliovirus 2A protease induced cleavage of the p220 component of eukaryotic initiation factor 4F Wyckoff, E. E.;Hershey, J. W. B.;Ehrenfeld, E.
  85. Virology v.82 Involvement of viral procapsid in the RNA synthesis and maturation of poliovirus Yin, F. H.
  86. Virology v.166 Protein 3CD is the major poliovirus responsible for cleavage of the p1 capsid precursor Ypma-Wong, M.-F.;Dewalt, P. G.;Johnson, V. H.;Lamb, J. G.;Semler, B. L.