Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Acute Toxicity Effect of Triotganotin on the Growth of Microalgae and Shellfish and A Design of A Chemostat System for the Chronic Toxicity Experiment

미세조류와 패류의 성장에 미치는 Triorganotin의 급성 독성영향 및 만성독성 실험을 위한 Chemostat System의 설계

  • 탁건태 (부경대학교 생물공학과) ;
  • 이형호 (부경대학교 생물공학과) ;
  • 홍용기 (부경대학교 생물공학과) ;
  • 김중균 (부경대학교 생물공학과)
  • Published : 1997.12.01
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Abstract

The acute toxicity effect of triorganotin of trioganotin on the growth of microalgae and shellfish was investigated through flask culture. The value of 120 hr-LC$_{50}$ that is the median lethal concentration of TBTO on the shellfish (R. philipinarum) was found to be 6 $\mu$g/L. The acute toxicity effect of TBTO on T. suecica was obviously shown even at the concentration of 0.5 $\mu$g/L, and the effect diminished as the initial cell density increased. The effect also diminished less in the experiment done under aeration than in that done under non-aeration. To design a chemostat system for the test of chronic toxicity, the culture of T. suecica was executed in photobioreactor. In batch culture, the profiles of chlorophyII a and D.C.W. showed the growth of T. suecica very well, and the maximum specific growth rate was estimated to be 0.54 d$^{-1}$. with this value, as a dilution rate in contimuous culture, pH was nicely maintained between 7 and 9 when air was supplied with 3% CO$_{2}$. From all results and the natural environment of clam, a novel chemostat system was invented. Through this system, we can observe each independent toxicity effect of TBTO and plankton and combined toxicity effect as well.

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References

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  18. 양식개론 유성규