Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지

Effects of Silver lon Exchanged Water Treatment Agent upon E. Coli RB 797 and Bacillus sp.

수처리제 은이온이 E. Coli RB 797과 Bacillus sp. 에 미치는 영향

  • 신혜자 (동서대학교 응용공학부 환경공학전공) ;
  • 신춘환 (동서대학교 응용공학부 환경공학전공)
  • Published : 1997.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of the silver ion-exchanged water treatment agent (Ag-Os) upon E. coli RB 797 and Bacillus sp. have been discussed in this study. Silver ion causes a number of toxic effects with no known biological function. Silver ion-exchanged water treatment agent (Ag-Os) using oyster shell here showed antimicrobial activities. the soluble form of silver ion in water is more toxic to the growth of Bacillus sp. than that of E. Coli RB 797. The minium amount of Ag-Os needed for growth inhibition is 0.2 mg/ml for E. Coli RB 797 and 0.02 mg/ml for Bacillus sp., which is consistant with the data of the survival cell fraction. Binding studies suggested that binding of silver to the cell surface was a rapid, metabolic-independent process and different from active transport. Bacillus sp. showed more binding than E. Coli RB 797. Reducing substances of the cell cultures in the presence of Ag-Os was detected using Methylen blue as an indicator. From these results, we suggest that Ag-Os is effective as an antimicrobial agent on E. Coli RB 797 and Bacillus sp. and silver binds to the cells through rapid, metabolic-independent process and might complex to sulfur group in the cells for its toxicity.

본 연구에서는 은이온이 이온 교환되어 있는 수처리제(Ag-Os)의 영향을 Bacillus sp. 와 E. Coli RB 797을 사용하여 연구하였다. Bacillus sp.의 성장이 E. Coli RB 797의 성장에서보다 더 은이온에 민감하게 억제됨을 보였다. 성장억제에 필요되어지는 Ag-Os양은 0.2 mg/ml 이상에서 E. Coli RB 797를 0.02 mg/ml 이상에서 Bacillus sp. 의 성장을 저해하며 Ag-Os 수처리제의 존재하에서 생존 할 수 있는 세포 수도 E. Coli RB 797이 더 많음을 보여 윗 결과와 일치함을 보였다. 세포에 bind되는 것은 몇 분안에 일어 나는 과정이며 starved cells에서도 일어나는 에너지를 필요치 않는 과정임을 Binding연구는 나타내고 있다. 또한 Bacillus sp.의 은이온 binding이 더 많이 일어남을 보여준다. 수처리제의 존재하에서 reducing substances가 생성됨을 methylene blue를 indicatr로 사용하여 관찰하였다. 이상의 결과로 이 수처리제는 E. Coli RB 797과 Bacillus sp. 에 대해 효과적이며 은이온은 빠르고 에너지를 필요로 하지 않는 과정에 의해 세포에 bind한후 세포내로 들어가 sulfur group과 반응할 것으로 사료된다.

Keywords

References

  1. Prog. Med. Chem. v.31 Antimicrobial activity and action of silver Russell, A. D.;Hugo, W. B.
  2. Appplied and Environmental Microgiology v.35 Reversal of the silver inhibition of microorganisms by agar. Tilton, R. C.;Rosenberg, B.
  3. Enzyme an dMicrobial Technology v.9 Silver resistance and accumulation in bacteria Trevors, J. T.
  4. Biol. Pharm. Bull. v.16 Effect of preinduction of metallothionein on tissue distribution of silver an hepatic lipid peroxidation Shinogi, M.;Maeizumi, S.
  5. J. Biol. Chem. v.270 Copper and silver transport by CopB-ATPase in membrane vesicles of Enterococcus hirae Solioz, M.;Odermatt, A.
  6. Biol. Met. v.3 silver dinsing to pseudomonas aeruginsa azurin Tordi, M. G.;Naro, F.;Giodano, R.;silvestrini, M. C.
  7. Nature v.296 Bacteria accumulate silve during leaching of sulphideore minerals Pooley, F. D.
  8. J. of Bateriology v.158 Plasmid-determined silver resitance in Pseudomonas atutzeri isolated from a silver mine Haefeli, C.;Franklin, C.lHardy, K.
  9. Moecular Biology, pathogenicity, and ecology of bacterial plasmids Mechanisms of plasmid-determined heavy metal resistances. Silver, S.;Levy, S. B.(et al. eds.)
  10. J. Clinical Pathology v.29 Instability an linkage of silver tesistance, lactose fermentation and colony structure in Enterobacter cloacae from burn winds Anner, D. I.;Mee, B. J.;Baily, M.
  11. British Medical Journal v.1 gentamicin- and silver-resistant pseudomonas in a burns unit Bridges, K.;Kidson, A.;Lowbury, E. J. L.;Wilkins, M. D.
  12. Biometals v.7 Plasmid mediated silver resistance in Acinetobacter baumannii Deshpande, L. M.;Chopada, B. A.
  13. Proceedings of the (38th) General Meeting of the Society for Industrial Microbiology v.23 Silver resistance in microoranisms. in Developments in industrial microbiology Belly, R. T.;Kydd, G. G.
  14. Experiments in cell physiology Parker, L.
  15. Biochemical tests for idemtification of medical bacteria MacFassin, J. F.
  16. J. Med. Microbiol v.29 Silver resistance in Escherichia coli R1 Starodub M. E.;Trevors, J. T.
  17. Biotechnology, Principles and applications.Blackwell Science Publications Materials and biotechnology Brierlsy, C. L.;Kelly, D. P.;Seal, K. J.;Best, D. J.;Higgins, J.(et al. eds.)
  18. FEMS Microbiology Review v.32 Metal resistance in bacteria Trevors, J. T.;Oddie, K. M.;Belliveau, B. H.