수수에서 식물호르몬 지베렐린의 circadian 리듬

Circadin Gibberellins production in Sorghum

  • 이인중 (경북대학교 농과대학 농학과) ;
  • 김길웅 (경북대학교 농과대학 농학과) ;
  • 김경민 (경북대학교 농과대학 농학과)
  • 발행 : 1997.09.01

초록

수수에서 식물호르몬 에틸렌 생합성은 circadin 리듬 현상을 보인다고 이미 보고된바 있다. 따라서 본 실험은 또 다른 식물호르몬 지베렐린 생합성도 에틸렌과 같은 circadin 리듬 현상을 보이는지를 조사한 결과를 요약하면 다음과 같다. 일정기간 동안 12시간 주기의 밤과 낮 조건에서 생육시킨 식물체를 연속광조건으로 옮긴후 매 시간 마다 지베렐린의 함량변화를 조사하였다. 1. GA$_{12}$는 공시한 두 품종 모두에서 뚜렸한 circadin 리듬현상을 보였다. 2. GA$_{53}$과 GA$_{19}$는 일체의 리듬현상을 보이지 않았다. 3. GA$_{20}$과 GA$_{1}$은 circadin 리듬현상을 보였지만 연속광하에서 리듬의 정도는 12시간 주기의 정상적인 일장하에 비해 현저히 감소하였다. 4. 수수의 개화는 원적외선광의 조사에 의해 촉진됨에도 불구하고 이들 처리는 내생지베렐린의 함량을 변화시키지 않았다.

The possibility of circadian production of plant hormone gibberellin (GA0 was examined in phytochrome B mutant (plyB-1) and wild-type sorghum. GA$_{12}$, GA$_{20}$ and GA$_{1}$ levels were found to cycle circadianly in both phyB-1 and wild-type. The periods (33 h) of GA$_{20}$ and GA$_{1}$ cycling in constant light were longer than normal photoperiods in both genotypes and typical average free running periods in plants of 22 to 28 h. The biological clock was thus shown to function properly in phyB-1. However, circadian regulation of GAs productions were not clear as compared to circadian ethylene regulation reported by Lee (1996). Although, in sorghum, EOD FR treatment hasten floral inititation, the differences in GA concentrations between treatments and untreated control were generally less dramatic than expected. Thus, it can be concluded that FR does not act primarily by changing absolute levels of GAs but rather by increasing flowering responsiveness to GAs.

키워드

참고문헌

  1. Plant. Physiol. v.99 Genetic regulation of development in Sorghum bicolor. Ⅶ. $ma_3^R$ flowering mutant lacks a phytochrome that predominates in green tissue. Childs, K. L.;Cordonnier-Pratt, M. M.;Pratt, L. H.;Morgan, P. W.
  2. Plant Physiol. v.100 Photophysiology of the elomutant lacks a detectable phytochrome B-like polypepride. Devlin, P. F.;Rood, S. B.;Sormers, D. E.;Quail, P. H.;Whitelam, G. C.
  3. Plant Cell. v.4 The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome. Lopez-Juez, E.;Nagatani, A.;Tomizawa, K. I.;Deak, M.;Kendrick, R. E.;Furuya, M.
  4. Plant Cell v.5 Mutations in the gene for the red/far red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Reed, J. W.;Nagpal, P.;Poole, D. S.;Furuya, M.;Chory, J.
  5. Plant Physiol. v.107 Phytochrome, gibberllin, and hypocotyl growth growth. A study using the cucumber (Cucumis sativus L.) long hypocotyl mutant. Lopex-Juez, E.;Kobayashi, M.;Sakurai, A.;Kamiya, Y.;Kendrick, R. E.
  6. Plant Physiol. v.108 Genetic regulation of development in Sorghum bicolor. Ⅸ. The $ma_3^R$ allele disrupts diurnal control of gibberellin biosynthesis. Foster, K. R.;Morgan, P. W.
  7. Plant Physiol. (Submitted). Photoperiodic control of gibberellin metabolism and flowering in a phytochrome B mutant of Sorghum bicolor. Lee, I. J.;Foster, K. R.;Morgan, P. W.
  8. Ph. D. Thesis, Texas A&M Univ. Photoperiodic control of gibberellin metabolism, flowering and circadian ethylene production in a phytochrome B mutant of Sorghum bicolor. Lee, I. J.
  9. Plant Physiol. v.104 Genes encoding glycine-rich Arabidopsis thaliana protein with RNA-binding motifs are influenced by cold treatment and an endogenous circadian rhythm. Carpenter, C. D.;Kreps, J. A.;Simon, A. E.
  10. Plant J. v.5 A light- and temperature-entrained circadian clock controls expression of transcripts encoding nuclear proteins with homology to RNA-binding proteins in meristematic tissue. Heintzen, C.;Melzer, S.;Fisher, R.;Kappeler, S.;Apel. K.;Staiger, D.
  11. Plant Mol. Biol. v.23 Carcadian and light-regulated expression of nitrate reductase in Arabidopsis. Pilgrim, M. L.;Casper, T.;Quail, P. H.;McClung, C. R.
  12. Plant Physiol v.103 Differential involvement of the circadian clock in the expression of genes required for ribulose-1,5-bisphosphate carboxylase/ oxygenase synthesis, assembly, and activation in Arabidopsis thaliana. Pilgrim, M. L.;McClung, C. R.
  13. Mol. Gen. Genet. v.237 Concerted circadian oscillations in transcript leveles of nineteen Lha/b(cab) genes in Lycopersicon esculentum (tomato). Kellmann, J. W.;Merforth, N.;Wiese, M.;Pichersky, E.;Piechulla, B.
  14. Planta v.165 Diurnal and circadian rhythmicity in the expression of light-induced plant nuclear messenger RNAs. Kloppstech, K.
  15. L. Kor. J. Crop Sci. (Submitted). Effect of gibberelin and phytochrome B on internode length of Sorghum bicolor Lee, I. J.;Kim, K. U.;Morgan, P. W.
  16. The physiological clock Bunning, E.
  17. Plant Physiol. v.108 Genetic regulation of development in Sorghum bicolor. X. Greatly attenuated photoperiod sensitivity in a phytochrome-deficient sorghum possessing a biological clock but lacking a a R-HIR. Childs, K. L.;Lu, J. -L.;Mullet, J. E.;Morgan, P. W.
  18. L. Plant. Physiol. v.82 Evidence for phytochrome regulation of gibberellin $A_{20}$ 3-hydrpxylation in shoots of dwarf(lele) Pisum sativum Campell, B. R.;Bonner, B. A.