생명과학회지 (Journal of Life Science)

  • 제7권3호
  • /
  • Pages.180-185
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  • 1997
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

Methylelaiophylin의 항균활성

Antibacterial Activities of Methylelaiophylin

  • 이동선 (경북대학교 미생물학과) ;
  • 이상한 (경북대학교 미생물학과) ;
  • 우주형 (경북대학교 미생물학과) ;
  • 이진만 (경북대학교 미생물학과) ;
  • 서영배 (경북대학교 미생물학과) ;
  • 홍순덕 (경북대학교 미생물학과)
  • 발행 : 1997.09.01
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초록

Methylelaiophylin은 gram양성균에 대하여 광범위한 항균활성을 보이며, 특히Bacillus subtilis에서 superoxide redicals을 발생하여 항균활성을 나타낸다. B. subtilis에 대한 항균활성은 wild type strain보다 mutant strain인 rec$^{-}$에 대하여 더민감하며, 이러한 항균력은 항산화제인 dithiothreito에 의하여 현저히 감소되었다. 또한 항균력은 세포내 RNA합성보다 DN합성에 효과적으로 작용하였다. 이러한 결과들은 methylelaiophylin의 항균활성이 세포내에서 발생한 활성산소에 의하여 기인한 것임을 나타낸다.

Methylelaiophylin generated superoxide radicals in Bacillus subtilis and showed antibacterial activity against a broad range of gram positive bacteria. The inhibition of DNA synthesis was more sensitive than one of RNA synthesis. A recombination-deficient mutant strain of B. subtilis was 2-fold more sensitive than a wild strain, and this sensitivity was reduced in the presence of an antioxidant, dithiothreitol. Methylelaiophylin generated superoxide radicals in B. subtilis lysates, and this suggests that the antibacterial activity of methylelaiophylin is related to the generation of active oxygen species in the cells.

키워드

참고문헌

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