Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of ozone treatment on the expression of a foreign gene in transgenic poplar subclones

형질전환 포플라 subclone의 도입유전자 발현에 대한 오존처리의 영향

  • 설일환 (경북대학교 농업과학기술연구소) ;
  • 신동일 (대구효성카톨릭대학교 자연대학 식물육종학과)
  • Published : 1997.09.01
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Abstract

Transgenic hybrid poplar subclones containing herbicide glyphosate resistant gene (aroA) were treated with ozone at the concentration of 100 nL L$^{-1}$ for 6 hr for 5 consecutive days. The foreign gene expression in leaves of all treated plants was reduced both at transcriptional and translational levels confirmed by Northern and Western blot analysis, respectively, as compared to non-treated control plants. These results indicated that the expression of foreign gene in transgenic plants could be affected by the environmental stresses. Thus, the performance of transgenic plants cultivated on field conditions may be lower than they are expected.

Keywords

References

  1. Plant Physiol v.93 Factors influencing the tissue culture and the Agrobacterium tumefaciens-mediated transformation of hybrid aspen and poplar clones. Block, M. D.
  2. Plant Mol. Biol. v.17 cDNA cloning sequence analysis and seasonal expression of lignin-bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase of aspen. Bugeos, R. C.;Chiang, V. L. C.;Campbell, W. C.
  3. Proc. Natl. Acad. Sci. v.86 Systemic accumulation of specific mRNAs in response to wounding in poplar trees. Parsons, T. J.;Bardshaw, J. H. D.;Gordon, M. P.
  4. Woody Plant Biotechnology Producing herbicide-tolerant Populus using genetic transformation mediated by Agrobacterium tumefaciens C58 : A summary of recent research Riemenschneider, D. E.;Haissig, B. E.;Ahuja(ed.)
  5. Can. J. For. Res. v.24 Transgenic larch expressing genes for herbicide and insect resistance. Shin, D. I.;Podia, G. K.;Huang, Y.;Karnosky, D. F.
  6. Can. J. For. Res. v.21 Natural selection for ozone tolerance in Populus tremuloides : an evaluation of nationwide trends. Berrang, P.;Karnosky, D. R.;Bennett, J. P.
  7. Environmental pollution v.50 Atomospheric ozon : Formation and effect on vegetation. Krupta, W. C.;Manning, W. J.
  8. Nature v.317 Expression in plants of a mutant aroA gene from Salmonella typhymurium confers tolerance to glyphosate. Comai, L. D.;Facciotti, D.;Hiatt, W. R.;Thompson, G.;Rose Re;Stalker, D. M.
  9. Mol. Gen. Genet v.206 Agrobacterium-mediated transformation and regeneration of Populus. Fillatti, J. J.;Kiser, J.;Sellmer J.;McCown, B. H.;Haissig, B. E.;Comai, L.
  10. Can. J. For. Res. v.6 Threshold levels for foliar injury to Populus tremuloides by sulphur dioxide and ozone. Karnosky, D .F.
  11. Molecular cloning. A laboratory manual Molecular cloning. A laboratory manual Sambrook, J.;Fritsch, E. F.;Maniatis, T.
  12. USDA For. Serv. General echnical Report NC-146 Biblography of Populus cell and tissue culture. Ostry, M. E.;Ward, K. T.
  13. Amer. J. Bot. v.79 Effect of ozone on chlorophyll content in Populus tremuloides. Gagnon, Z. E.;Kamosky, D. F.;Dickson, R. E.;Isebrands, J. G.
  14. Michigan Tech. Univ. Effects of ozone on gene expression in sensitive and tolerant aspen clones. MS thesis Sheng, Y.
  15. Plant Physiol. v.96 Response of photosynthesis and cellular antioxidants to ozone in Populus leaves. Gupta, A. S.;Alsher, R. F.;McCune, D.
  16. Plant Physiol. v.104 Biochemical plant responses to ozone. Ⅳ. Cross-induction of defensive pathways in parsley (Petroselinium crispum) plants. Eckey-Kaltenbach, H.;Ernst, D.;Heller, W.;Sandermann, J. H.
  17. Annu. Rev. Plant Physiol. v.33 Oxyen activation and oxgen toxicity. Elstner, E. F.