생명과학회지 (Journal of Life Science)

  • 제7권3호
  • /
  • Pages.167-171
  • /
  • 1997
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

이중선RNA결합담백질(RBFII)의 cDNA분리

Isolation of cDNA Encoding Double-Stranded RNA Binding Protein (RBFII)

  • 박희성 (대구효성카톨릭대학교 식물육종학과)
  • 발행 : 1997.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

번역개시 및 인산화의 조절에 관여하는 RNA와 단백질의 결합 및 인식기작을 연구하기 위해서[$\alpha$^{-32}$P] UMP-labeled HIV Rev-responsive element(RRE) RNA를 이용한 affinity screening에 이해서 Hela ZA-PII cDNA library로부터 이중선RNA결합단백질의 cDNA (RBFII)를 분리하였다. RBFII의 cDNA에 대한 염기서열을 결정하였으며 기존에 연구된 바 있는 RBFII(RBF 또는 TRBP로 보고되었으며 본 연구에서는 RBFII와 구분하기 위해 RBFI으로 명명)과 대부분의 경우 공통적인 ORF를 지니는 것으로 나타났다. 그러나 5’말단에서는 공통적인 ORF 가 RBFI의 경우 21개의 아미노산을 의미하는 63 nt가 Lac-Z의 N-말단에 연결된데 비해서 특이한 46개 아미노기를 의미하는 138nt가 존재함이 밝혀졌다. 5’-말단에 처음 나타나는 ATG 및 부근의 염기서열을 분석해 볼 때 양 cDNA는 5’말단이 완전하지 않은 것으로 사료된다.

As an initial effort to elucidate RNA: protein binding in a way to regulate translation initiation and phosphorylation, a cDNA encoding a double-stranded RNA binding factor (RBFII)was isolated from Hela ZAPII cDNA library by affinity screening using [$\alpha$$^{-32}$P] UMP-labeled HIV Rev-responsive element(RRE) RNA. The nucleotide sequence of RBF (or TRBP) cDNA except the 5’end. At the 5’end, This common ORF was fused in-frame to N-terminal residues of Lac-Z through a unique 138 nt sequence encoding 46residues in the case of RBFII and a 63 nt sequence encoding 21 residuces in the case of RBFI. The context of ATG appearing first in the sequences suggests that both these cDNA inserts are incomplete at the 5’end.

키워드

참고문헌

  1. Genes & Dev. v.3 RNA-binding proteins as developmental regulators. Bandziulis, R. M.;Swanson, M. S.;Dreyfuss, G.
  2. Mol. Biol. Rep. v.14 A seletive revies of RNA-protein interactions in eukaryotes. Mattaj, I. W.
  3. Cell v.59 Tat trans-activates the human immunodeficiency virus through a nascent RNA target. Berkout, B.;Silverman, R. H.;Jeang, K. T.
  4. Cell v.59 Seauence-specific recognition of RNA hairpins by bacteriophage antitermination requires a conserved arginine-rich motif. Lazinski, D. E.;Grzadzielska, E.;Das, A.
  5. Mol. Cell. Biol. v.11 Molecular analysis of two poly(A) site-processing factors that determine the recognition and efficiency of cleavage of the premRNA. Gilmartin, G.;Nevins, J.
  6. Science v.240 Iron-responsive elements : regulatory RNA sequences that control mRNA levels and translation. Casey, J. L.;Hentze, M. W.;Koeller, D. M.;Caughman, S. W.;Rouault, T. A.;Klausner, R. D.;Harford, J. B.
  7. Cell. v.68 Phosphorylation of initiation factor 2 alpha by protein kinase GCN2 mediates gene-specific translational control of GCN4 in yeast. Dever, T. E.;Feng, L.;Wek, R. C.;Cigan, A. M.;Conahue, T. F.;Hinnenbusch, A. G.
  8. Nucleic Acids Res. v.19 Detailed mutational analysis of TAR RNA : critical spacing between the bulge and loop recognition domains. Berkout, B.;Jeang, K. T.
  9. DAI. Genes & Dev. v.6 Two RNA binding motifs in the double-stranded RNA activated protein kinase Green, S. R.;Mathews, M. B.
  10. Eur. J. Biochem. v.178 The binding of double-stranded RNA and adenovirus VAI RNA to the interferon-induced protein kinase. Galabru J.;Katze, M. G.;Robert, N.;Hovanessian, G.
  11. Virology v.172 Mechanism of interferon action. Activation of the human P1/eIF-2 alpha protein kinase by individual reovirus s-class mRNAs : s1 mRNA is a poten activator relative to s4 mRNA. Bischoff, J.;Samuel, C. E.
  12. EMBO J. v.11 Human p68 kinase exhibits growth suppression in yeast and homology to the translational regulator GCN2. Chong, K. L.;Feng, L.;Schappert, K.;Meurs, E.;Donahue, T. F.;Friesen, J. D.;Hovanessian, A. G.;Willians, B. R.
  13. Proc. Natl. Acad. Sci. USA. v.91 TAR RNA-binding protein is an inhibitor of the interferon-in-duced protein kinase PKR. Park, H.;Davies, M. V.;Langland, J. O.;Chang, H.-W.;Nam, Y. S.;Tartaglia, J.;Paoletti, E.;Jacobs, B. L.;Kaufman, R. J.;Venkatesan, S.
  14. Park, H.;Choi, J.
  15. Kor. J. Gen. v.19 Inhibition of PKR phosphorylation in vitro by Lac-Z fused double-stranded RNA blinding protein (RBF) produced from E. coli. Park, H.;Choi, J.
  16. J. Virol. v.64 Human immunodificiency virus rev protein recognizes a target suquence in revresponsive element RNA within the context of RNA econdary structure. Holland, S. M.;Ahmad, N.;Maitra, R. K.;Wingfield, P.;Venkatesan, S.
  17. Proc. Natl. Acad. Sci. USA. v.74 DNA sequencing with chain-terminating inhibitors. Sanger, F.;Nicklen S.;Coulson, A. R.
  18. Science v.251 Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR. Gatignol, A.;Buckler-White, A. J.;Berkhout, B.;Jeang, K.-T.