Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지

Distribution of Phyrase in The Development Rat Organs

성장 발육에 따른 흰쥐 장기내 phytase의 분포성

  • 양원진 (동아대학교 유전공학연구소)
  • Published : 1997.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The phytase(myo-inositol hexkisphosphate phosphohydrolase ; EC 3.1.3.8) activity was observed only in the homogenate of intestinal mucosa, though the activity of alkaline phisphatase was measurable in various organs. In addition, no protein bands were detected in any other organs on immunoblotting using the anti-90kDa phytase antiserum. Thses results suggest that phytase is specifically present in small intestinal mucosa, and that hydrolysis of phytic acid(inositol-hexakisphosphate) can be allotted for a physiological role of the intestine-specific enzyme. The activities of phytase was increased during development of rat. The 70kDa phytase appeared just after birth, but the 90kDa phytase was not observed until adult period, suggesting that the 90kDa phytase was synthesized in response to weanling.

Phytase(myo-inositol hexakisphosphate phosphohydro-lase;EC 3.1.38)활성은 소화기관인 소장 점막에서나 나타났고, 그 외 다른 장기에서는 alkaline phosphatase활성만을 측정할 수 있었다. 그리고 anti-90kDa phytase항혈청을 이용한 면역조직학적 조사 결과 소장 이외의 장기에서는 본 효소의 단백질 밴드가 검출되지 않았다. 이와 같은 결과 phytase는 소장에만 특이적으로 존재하며, 소장 특이적 효소의 생리적 역할로서 Phytic acid(inositol=hexakisphos-phate)를 가수분해한다. 흰쥐의 성장 발육과 더불어 phytase의 활성은 증가한다. 출생 후부터 이유기 전까지는 70kDa phytase외에도 90kDa phy-tase가 출현한다. 이 90kDa phy-tase는 이유기에 합성되는 것으로 추정된다.

Keywords

References

  1. Adv. Food Res. v.28 Phytates in legumes and cereals Reddy, N. R.;Sathe, S. K.;Sakunkhe, D. K.
  2. J. Agric Food Chem. v.30 Phytate its chemistry, occurrence, food interactions, nutritional significance and methods of analysis Maga, J.
  3. 情報處理 v.35 腦ガ食べる-代謝をめぐる諸問題 中川八郎
  4. Ann. Rev. Nutr. v.6 metabolism and function of myo-inosi-tol and inositol phospholipids Holub, B. J.
  5. Biochim. Biophys. Acta v.929 purification and properties of inositol-1, 4-bisphosphate 4-phcsphohydrolase from rat brain Takimoto, K.;Motoyama, N.;Nakagawa, H.
  6. J. Biochem. v.106 purification and characterizationof membrane-bound inositolpolyphosphate 5-phosphatase Takimoto, K.;okada, M.;nakagawa, H.
  7. Biochem. J. v.31 Patwardhan, V. N.
  8. Biochim. Biophys. Acta v.268 phytase and alkaline phosphatase activities in intestinal mucosa of rat, chicken, Calf and man Bitar, K.;Reinhold, J. G.
  9. Gut. v.24 Distribution and properties of CaATPase(EC 3.6.1.3) phytase(EC 3.1.3.8) and alkaline phosphatase(EC 3.1.3.1) in isolated enterocytes from normal and vitamin D deficient rats Chan, S. D.;Atkin, S. D.
  10. Comp. Biochem. Physiol. v.42B Intestinal alkaline phosphatase and phytase of chicks : Separation of isoenzymes, zinc contents and in vitro effects of zinc Davis, M. I.;Motzok, I.
  11. Br. J. Nutr. v.39 The similarity between alkaline phosphatase (EC 3.1.3.1) and phytase(EC 3.1.3.8) activities inrat intestine and their importance in phytate-induced zinc deficiency Davis, N. T.;Flett, A. A.
  12. Arch. Biochem. Biophys. v.260 Purification and characterization of phytase from cotyledons of germinating soybean seeds Gibson, D. M.;Ullah, A. H. J.
  13. Br. J. Nutr. v.54 A comparative study of phytate hydrolysis in the gastrointestinal tract of the golend hamster mesocricetus-auratus and the laboratory rat Williams, P. J.;Taylor, T. G.
  14. Arch. Biochem. Biophys. v.241 The relationship of alkaline phosphatase CaATPase and phytase Williams, S. A.;Culp, J. S.;Butler, L. G.
  15. ラット小腸粘膜のPhytaseとAlkaline Phosphatase v.61 no.9 梁元鎭 ;松田 宏;中川八郎
  16. 中川八郎 ラットの生育及び食餌條件に伴う小腸フィ夕-ゼ (小腸型アルカリ性フォスファ -ゼ)のサブユニット構造の變化 v.62 no.7 松田義宏;梁元鎭;中川八郎
  17. Biochim. Biophys. Acta. v.1075 Purification and characterizationof phytase from rat intestinal mucosa Yang, W. J.;Mastuda, Y.;Sano, S.;masutani, H.;nakagawa, H.
  18. Biochim. Biophys. Acta. v.1075 Developmental and dietary induction of the 90K subunit of rat intestinal phytase Yang, W. J.;mastuda, Y.;Inomata, M.;Nakagawa, H.
  19. Korean. Biochem. J. v.27 no.4 Kinetic Properties of Rat Intestinal Phytase/Alkaline Phosphatase Yang, W. J.;Kim, K. W.
  20. Arch. Pham. Res. v.17 no.5 Characterization of oligosaccharide moieties of rat intestinal phytase Yang, W. J.;Kim, K. W.
  21. 생명과학지 v.7 no.2 흰쥐 소장 점막 Phytase의 특성 및 활성에 미치는 금속 이온의 영향 양원진;손홍대
  22. Anal. Biochem. v.113 A new and convenient colorimetric determination of norganic ortho-phosphate and its application to the assay of inorganic phrophosphatase Heinonen, J. K.;Lahti, R. J.
  23. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O. H.;Rosebrough, N. J.;Farr, A. L.;Randall, R. J.
  24. Nature. v.227 cleavage of structural proteins during the assembly of the head ofacteriophage T4 Laemmli, U. K.
  25. Science v.211 Ultrasensitive stain for proteins in polyacry-lamide gels shows regional variation in cerebrospinal fluid proteins Merril, C. R.;Goldman, D.;Sedman, S. A.;Ebert, M. H.
  26. Anal. Biochem. v.109 Elution of Proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity : results with sigma subunit of Escherichia Coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes Hagar, D. A.;Burgess, R. R.
  27. J. Biochem. v.105 A novel brain-specific antigen : a glycoprotein electrophoretically similar to but immunochemically different from type B nucleoside diphosphatase Sano, S.;Mastuda, Y.;Nakagawa, H.
  28. Proc. Natl. Acad. Sci. U. S. A. v.76 Electro-phoretic transfer of proteins from polyacrylamide gels to nitrocellurose sheets : Procedure and some applications Towbin, H.;Staehelin, T.;Gordon, J.
  29. J. Biol. Chem. v.260 Isozymes of bovine intestinal alkaline Phosphatase Besman, M.;Coleman, J. E.
  30. Br. J. Nutr. v.49 Adaptive increase in phytate digestibility by phosphorus-deprived rats and relationship of intestinal phytase(EC 3.1.3.8)and alkaline phosphatase(EC 3.1.3.1) to phytate utilization Moore, R. J.;Veum. T. L.
  31. J. Nutr v.118 Effect of dietary phytase on the digestion of phytate in the stomach and small intestine of humans Sandberg, A. -S.;Andersson, H.