생명과학회지 (Journal of Life Science)

  • 제7권2호
  • /
  • Pages.112-118
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  • 1997
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

회전원판 반응조를 이용한 Pseudomonas sp. KS-96에 의한 gallic acid로부터 Pyrogallol의 전환

Bioconversion of Pytogallo from Gallic Acid by Pseudomonas sp. KS-96 using Rotating Disc Contactor

  • 발행 : 1997.06.01
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초록

Gallic acid로부터 pyrogallol로 전환하는 균주를 토양에서 분리 동정하여 얻는 Pseudomonas sp. KS-96의 균체를 회전원판 반응조에 고정화시킨 후 연속전환을 시도하여 다음과 같은 결과를 얻었다. Gallate decarboxylase의 활성은 배양 24시간에서 가장 좋았다. 반응조의 원판에 gallic acid가 함유된 액체 배양액을 시간당 20m${\ell}$씩 공급하면서 균체막의 두께가 steady state에 도달할때까지 공급하였다. 균체를 고정화 시킨 후 시간 경과에 따른 전환율은 18시간부터 36시간에서 전환율이 높았다. 균체 고정화 후에 gallic acid를 15g/${\ell}$ 함유하는 기질을 시간당 30m${\ell}$씩 주입했을 때 pyrogallol의 전환율이 가장 좋았으며 회전원판 반응조에 의한 연속적 전환은 6일에서 8일까지가 각각 81%, 80%로서 가장 높았다.

In previous paper Pseudomonas sp. Ks-96 isolated from the bioconversion into pyrogallol from gallic acid . Continuous bioconversion of pyrogallol was carried out using rotatory disc contactor immobilized Pseudomonas sp. Ks-96 . Enzyme activity of gallate decarboxylase released from Pseudomonas sp. Ks-96 were shown at the highest activity on 24h incubation. Culture media containing gallic acid supplied on the flow rate of 20m${\ell}$/h until thickness of cells wall reached steady state. Bioconversion rate of pyrogallol from gallic acid showed at highest level ranging from 18hr to 36h according to time courses. Continuous bioconversion of pyrogallol using rotating disc contactor was about 82% and 80% between 6 and 8 days at the feeding rate of 300m${\ell}$ per hour in the medium containing 15g/${\ell}$ gallic acid.

키워드

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