Journal of Microbiology

  • 제35권3호
  • /
  • Pages.234-238
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  • 1997
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

A conditional lethal mutation of a nucleoporin gene, NUP49 in saccharomyces cerevisiae

  • Lee, Youn-Soo (Department of Microbiology, Chungnam National University) ;
  • Song, Young-Ja (Department of Microbiology, Chungnam National University) ;
  • Kyung, Hwang-Mi (Department of Microbiology, Chungnam National University) ;
  • Lee, Woo-Bok (Department of Microbiology, Chungnam National University) ;
  • Kim, Jin-Mi (Department of Microbiology, Chungnam National University)
  • 발행 : 1997.09.01
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초록

Conditional lethal mutation nup49-1 of a nuclear pore complex component gene was constructed in Saccharomyces cerevisiae. This mutation deleted one third of the essential NUP49 gene at the carboxy-terminal, but retained 13 repeats of the highly conserved GLFG domain. The nup49-1 mutant strain was viable with a slow-growth phenotype, indicating that the C-terminal is dispensable at normal growth temperature. This strain exhibited both temperature-sensitivity at 37.deg.C and cold-sensitivity at 16.deg.C. Temperature shift experiments revealed that the arrest phenotype at 37.deg.C was random in the cell division cycle. The nup49-1 mutation was tested to be recessive and is expected to be useful for the functional analysis of nuclear pore complex proteins as well as for studies of nuclear transport systems.

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참고문헌

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