Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Nitro oxide in human cytomegalovirus replication and gene expression

  • Published : 1997.06.01
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Abstract

Infection of human fibroblast (HF) cells with human cytomegalovirus (HCMV) result in changes in the intracellular level of second messengers. Since nitric oxide (NO) production has been known to be related with other second messengers, it is probable that HCMV infection of HF cells may involve NO. To test this possibility, the amount of NO was measured following ogenous addition of NO generators such as sodium nitroprusside (SNP) or S-nitroso-N-a-cetylpenicillamine (SNAP) immediately after HCMV infection, however, inhibited virus multiplication. Furthermore, immunoblot experiment using monoclonal antibody to HCMV major immediate early (MIE) proteins or CAT assay using pCMVIE/CAT (plasmid containing CAT gene driven by HCMV MIE promoter) revealed that SNP or SNAP blocked the MIE gene expression. SNP was more effective than SNAP in hibiting HCMV multiplication or MIE gene expression. SNP produced more NO than SNAP in inhibiting HCMV multiplication or MIE gene expression. SNP produced more NO than SNAP. Although the mechanism for the inhibition of HCMV multiplication and MIE gene expression by NO is still elusive some correlation with NO-mediated inhibition of HCMV-induced increase in cytosolic free Ca$\^$2+/ concentration ([Ca$\^$2+/]) was observed. The increase of [Ca$\^$2+/] following HCMV infection was inhibited by SNP, and less effectively by SNAP. Raising [Ca$\^$2+/ with bromo-A23187 partially reversed the SNP block of MIE gene expression. Thus, there appear to e some relationships among NO. [Ca$\^$2+/], and HCMV MIE gene expression.

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References

  1. J. Virol. v.69 Inhibitory effect of nitric oxide on the replication of a murine retrovirus in vitro and in vivo Akarid, K.;M. Sinet;B. Desforges;M.A. Gougerot-Pocidalo
  2. J. Virol. v.61 Antigenic cross-reactions among herpes simplex virus types 1 and 2, Epstein-Barr virus, and cytomegalovirus Balachandran, N.;D.E. Oba;L.M. Hutt-Fletcher
  3. Adv. Cyclic Nucl. Pro. Phospho. Res. v.17 Regulation of cyclic GMP formation by soluble guanylate cyclase: stimulation by NO-containing compounds Bohme, E.;G. Grossmann;J. Herz;A. Mulsch;C. Spies;G. Schultz
  4. FEBS Lett. v.265 Calcium-dependent nitric oxide synthesis in endothelial cytosol is mediated by calmodulin Busse, R.;A. Mulsch
  5. Curr. Top. Microbiol. Immunol. v.154 Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169 Chee, M.S.;A.T. Bankier;S. Beck;R. Bohnl;C.M. Brown;R. Cerny;T. Horsnell;CoA. HutchisonⅢ;T. Kouzarides;J.A. Martignetti;E. Preddie;S.C. Satchwell;P. Tomlinson;K.H. Westen;B.G. Barnell
  6. Mol. Pharmacol. v.47 Nitric oxide modulation of agonist-evoked intracellular $Ca^{2+}$ release in neurosecretory PC-12 cells: inhibition of phospholipase C activity via cyclic GMP-dependent protein kinase Ⅰ Clementi, E.;I. Vecchio;C. Sciorati;G. Nistico
  7. J. Clin. Invest. v.91 Evidence for an antiviral effect of nitric oxide: inhibition on Herpes simplex virus type 1 replication Croen, D.K.
  8. Nature v.288 The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine Furchgott, R.F.;J.V. Zawadzki
  9. Amer. J. Physiol. v.267 Regulation of calcium mobilization and entry in human platelets by endothelium-derived factors Geiger, J.;C. Nolte;U. Walter
  10. J. Virol. v.69 Gamma interferon-induced, nitric oxide-mediated inhibition of vaccinia virus replication Harris, N.;R. Mark;L. Buller;G. Karupiah
  11. Amer. J. Physiol. v.266 Changes in cytosolic cGMP and calcium in airway smooth muscle relaxed by 3-morpholinosydnonimine Jones, K.A.;R.R. Lorenz;D.O. Warner;Z.S. Katusic;G.C. Sieck
  12. Mol. Cells v.3 Increased cytosolic free calcium concentration following HCMV infection of human embryo lung cells Kang, K.H.;C.H. Yoo;C.H. Lee
  13. Mol. Cells v.5 Effect of human cytomegalovirus immediate early gene expression on cytosolic free calcium concentration Kang, K.H.;C.H. Lee
  14. Science v.261 Inhibition of viral replication by interferon-γ-induced nitric oxide synthase Karupiah, G.;Q.-W. Xie;R.M.L. Buller;C. Nathan;C. Duarte;J.D. MacMicking
  15. Intervirology v.38 Nitric oxide inhibits Epstein-Barr virus DNA replication and activation of latent EBV Kawanishi, M.
  16. Mol. Pharmacol. v.41 Sodium nitroprusside inhibits N-methy-D-aspartate-evoked calcium influx via a nitric oxide- and cGMP-independent mechanism Kiedrowski, L.;E. Costa;J.T. Wroblewski
  17. Mol. Cells v.3 Increased cyclic AMP levels in human fibroblast cells infected with human cytomegalovirus Lee, C.H.;O.S. Steinsland;T. Albrecht
  18. Mol. Cells v.4 Protein kinases in human cytomegalovirus infection and gene expression Lee, C.H.;J.H. Yoon;K.H. Kang
  19. Biochem. Biophys. Res. Commun. v.179 Inactivation of ribonucleotide reductase by nitric oxide Lepoivre, M.;F. Fieschi;J. Coves;L. Thelander;M. Fontecave
  20. FEBS Lett. v.380 Inhibition of the skeletal muscle ryanodine receptor calcium release channel by nitric oxide Meszaros, L.G.;I. Minarovic;A. Zahradnikova
  21. Biochem. Biophys. Res. Commun. v.180 Phosphorylation by calcium calmodulin-dependent protein kinase Ⅱ and protein kinase C modulates the activity of nitric oxide synthase Nakane, M.;J. Mitchell;U. Forstermann;F. Murad
  22. J. Biol. Chem. v.269 Regulation of biosynthesis of nitric oxide Nathan, C.;Q-W. Xie
  23. Virology v.157 Calcium response in cytomegalovirus-infected fibroblasts of human origin Nokta, M.;D. Eaton;O.S. Steinsland;T. Albercht
  24. Nature v.333 Vascular endothelial cells synthesize nitric oxide from L-arginine Palmer, R.M. J.;D.S. Ashton;S. Moncada
  25. Biochem. Biophys. Acta v.1178 The nitric oxide and cGMP signal-transduction pathway Schmidt, H.H.H.W.;S.L. Lihmann;U. Walter
  26. Cell v.78 NO at work Schmidt, H.H.H.W.;U. Walter
  27. Sci. Amer. May Biological roles of nitric oxide Snyder, S.H.;D.S. Bredt
  28. J. Virol. v.65 Cytomegalovirus homologs of cellular G protein-coupled receptor genes are transcribed Welch, A.R.;L.M. McGregor;W. Gibson
  29. Proc. Natl. Acad. Sci. USA v.91 Cyclic nucleotide-gated channels: an expanding family of ion channels Yau, K.-W.