Characterization of a new staphylococcal site-specific recombinase sin and genetic organization of its flanking region

  • 발행 : 1997.06.01

초록

A new site-specific recombinase sin, as a component of a putatie transposon has been cloned and its base sequence has been determined. The proposed sin shows a hish degree of homology with pI9789-sin and pSK1-sin. There is a large (16 bp) inverted repeat downstream of proposed sin and the postulate dhelix-turn-helix motif is located at the extreme C-terminus of the poposed Sin. The transposase gene (tnpA) and .betha.-lactamase gene (blaZ) are located upstream of sin and arsenate reductase gene (arsC) and arsenic efflux pump protein gene (ars B) are downstream. This genetic arrangement seems to be a part of a new putative transposon because there is no known transposon with a gene arrangement of tnpA-blaZ-sin-arsC.

키워드

참고문헌

  1. Getics of Bacteria Bacterial Transposons Bennett, P.;J. Scaife(ed.);D. Leach(ed.);A. Galizzi(ed.)
  2. Proc. Natl. Acad. Sci. v.72 Transposition of R factor genes to bacteriophage λ Berg, D.E.;J. Davies;B. Allet;J.D. Rochaix
  3. Gene v.81 Nucleotide sequence analysis of IS256 from the Staphylococcus aureus gentamycin-tobramycin-kanamycin-resistance transposon Tn4001 Byrne, M.E.;Rouch, D.A.;R.A. Skurray
  4. Mol. Microbiol. v.17 Rearrangement in the staphylococcal β-lactamase encoding plasmid, pIP1066, including a DNA inversion that generates two alternative transposon Derbise, A.;K.G. Dyke;N. el Solh
  5. Kor. Jour. Microbiol. v.32 Genetic organization of an inducible β-lactamase gene isolated from chromosomal DNA of Staphylococcus aureus Kim, Y.S.;K.I. Min;W.-H. Byeon
  6. Ph. D. thesis. Seoul National University Structural and functional requirements for IS 50-specific recombination Chung, J.H.
  7. Mol. Gen. Genet. v.189 Protoplast transformation of Staphylococcus carnosus by plasmid DNA Gotz, F.;B. Kreutz;K.H. Schleifer
  8. Pro. Natl. Acad. Sci. v.79 DNA sequence organization of IS10-right of Tn10 and comparison with IS-left Halling, S.M.;R.W. Simons;J.C. Way;R.B. Walsh;N. Klckner
  9. J. Bacteriol. v.174 Regulation and expression of arsenic resistance operon From Staphylococcus aureus plasmid pI258 Ji, G.;S. Silver
  10. Pro. Natl. Acad. Sci. v.72 Site-specific recA-independent recombination between bacterial plasmid: involvement of palindromes at the recombinational loci Kopecko, D.J.;S.N. Cohen
  11. J. Bacteriol. v.176 Plasmid-borne cadmium resistance genes in Listeria monocytogenes are present on Tn5422, a novel transposon closely related to Tn917 Lebrun, M.;A. Audurier;P. Cossart
  12. Gene. v.141 Characterization of sin, a potential recombinase-encoding gene from Staphylococcus aureus Paulsen, I.T.;M.T. Gillespie;T.G. Littlejohn;O. Hanvivatvong;S. Rowland;K.G.H. Dyke;R. A. Skurray
  13. EMBO. J. v.8 Characterization of the staphylococcal β-lactamase transposon Tn552 Rowland, S.J.;K. G.H. Dyke
  14. Mol. Micro. v.4 Tn552, a novel transposable element from Staphylococcus aureus Rowland, S.J.;K.G.H. Dyke
  15. EMBO J. v.14 Tn552 transposase purification and in vitro activities Rowland, S.J.;D.J. Sherratt;W.M. Stark;M.R. Boocock
  16. Pro. Natl. Acad. Sci. v.74 Sequencing with chain termination inhibitors Sanger, F.;S. Nicklen;A.R. Coulson
  17. Anti. Agent. Chem. v.36 Sequence analysis of the β-lactamase repressor from Staphylococcus aureus and hybridization studies β-lactamase-producing isolates of Escherichia faecalis Smith, M.C.;E.M. Barbara
  18. FEMS Mocrobiol Lett. v.127 Interaction of the chromosomal Tn551 with two thermosensitive derivatives, pS1 and pδD, of plasmid pI9789 in Staphylococcus aureus Sohail, M.;M. Oldridge;K.G. Dyke
  19. Anti. Agent. Chem. v.37 Genes involved in the regulation of β-lactamase production in Enterococci and Staphylococci Zscheck, K.K.;B.E. Murray