Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Development of Anticancer Agents from Korean Medicinal Plants (Part 4). Antitumor Activity of the Butanol Soluble Fraction of Perilla frutescens

한국산 생약으로부터 항암물질의 개발(제4보) 소엽 부탄올 가용분획의 항암활성

  • 최규은 (원광대학교 자연과학대학 화학과) ;
  • 곽정숙 (목포전문대학 치위생과) ;
  • 김영옥 (원광대학교 자연과학대학 화학과) ;
  • 백승화 (원광대학교 자연과학대학 화학과) ;
  • 한두석 (원광대학교 치과대학 구강해부학실)
  • Published : 1997.12.01
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Abstract

This study was carried out to develop antitumor effect of the n-butanol soluble fraction of Perilla frutescens on (KB cells) human oral epitheloid carcinoma cells. The cytotoxictty of methanollc extract of Perilla frutescens on KB cells was evaluated by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) assay. The antitumor activity of various fractions obtained from n-butanol soluble fraction of Perilla frutescens was evaluated in human oral epithelold carcinoma cells. The antitumor acavity of the n-butanol soluble fraction on human oral epitheloid carcinoma cells was evaluated by MTT assay of colorimetric method. The light microscopic study was carried out to observe morphological changes of cultured human oral epitheloid carcinoma cells. These results were obtained as follows; 1. The fractions 1,2 and 3 of the n-butanol soluble fraction of Perilla frutescens were shown significant antitumor activities. 2. The number of human oral epitheloid carcinoma cells were decreased and tend to form cell cluster by treatment with fractions 1,2,3 and 4 of the n-butanol soluble fraction of Perilla frutescens. 3. The fraction 1 of the n-butanol soluble fraction of Perllla frutescens showed the highest antitumor activity on Perilla frutescens. It has been selected as a lead fraction for further examinations.

Keywords

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