Animal cells and systems
- 제1권4호
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- Pages.657-663
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- 1997
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- 1976-8354(pISSN)
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- 2151-2485(eISSN)
High-Level Constitutive Expression of Mouse CD4 and CD4/CD8${\alpha}$ Hybrid Molecules in Transgenic Mice
- Kim, Joongkyu (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University) ;
- Choi, Young-Il (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University) ;
- Park, Sang-D (Howard Hughes Medical Institute,the Rockefeller University) ;
- Seong, Rho-H (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University) ;
- 발행 : 1997.12.01
초록
The CD4 and CDS coreceptors, in conjunction with the T cell receptor (TCR) , make important contributions to the differentiation of thymocytes. They have been shown to be involved in the clonal deletion and positive selection processes during T cell development in thymus. To further analyze the role of CD4 and CDS proteins during T cell differentiation, we have generated transgenic mice constitutively expressing high levels of a native CD4 and a CD4{CDSa hybrid protein. The hybrid protein is composed of CD4 extracellular domain linked to the CD8a transmembrane region and cytoplasmic tail. The transgenes were driven by human beta-actin promoter, and therefore, they were expressed in all tissues examined including thymus, spleen, and lymph nodes. The resulting CD4 and CD4{CD8
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