Animal cells and systems

  • 제1권3호
  • /
  • Pages.463-466
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  • 1997
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

${\gamma}$-NGF Produced in CHO Cells Does Not Cleave Mouse Ren-2 Prorenin

  • Rhee, Hee-Sub (Department of Obstetrics & Gynecology, College of Medicine, Wonkwang University) ;
  • Jeon, Byung-Hoon (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Won-Sin (Division of Life Science, College of Natural Sciencs, Wonkwang University)
  • 발행 : 1997.09.01
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초록

We have recently demonstrated, by protein and cDNA sequence analysis, that prorenin converting enzyme (PRECE) in the mouse submandibular gland is identical to the epidermal growth factor-binding protein (EGF-BP)type B. However, type A and C did not show prorenin converting activity. To demonstrate whether r-NGF is involved in prorenin processing, we have cloned cDNA of r-NGF and examined prorenin converting activity using the CHO cell expression system, Trypsin converted the 33 kDa r-NGF precursor produced in CHO cells to a two-chain form, 9.4 and 16.4 kDa polypeptide chains, which has been known as an active form of r-NGF in mouse SMG (Server and Shooter, 1976). However, the two chain forms of r-NGF did not reveal prorenin-processing activity. Thus, only PRECE is involved in prorenin processing in mouse SMG. This result shows that their substrate specificities appear to be very strict, although some kallikreins share a high degree of amino acid sequence identity.

키워드

참고문헌

  1. Biochemistry v.28 Substrate specificities of growth factor associated kallikreins of the mouse submandibular gland Blaber M;Lsackson PJ;Marsters JC Jr;Burnier JP;Bradshaw RA
  2. Circ Res v.23 Isolation from a salivary gland of granules containing remim and kalikrein Chiang TS;Erdos EG;Miwa I;Tague LL;Coalson JJ
  3. Biochemistry v.26 Mouse glandular kallikrein genes: indentification and characterization of the genes encoding the epidermal growth factor binding proteins Drinkwater CC;Evans BA;Richards RI
  4. J Biol Chem v.262 Mouse Glandular Kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus Evans BA;Drinkwater CC;Richrds RI
  5. Biochemistry v.30 β- endopeptidase: structure and activity of a kallikrein encoded by the gene mGK-22 Fahnestock M;Woo JE;Lopez GA;Snow J;Walz DA;Arici MJ;Mobley WC
  6. Proc Natl Acad Sci USA v.76 The biosynthetic precursor of epidermal growth factor and the mechanism of its processing Frey P;Forand R;Maciag T;Shooter EM
  7. J Biochem (Tokyo) v.107 Purification of mouse Ren-2 proremim produced in chinese hamster ovary cells Hatsuzawa K;Kim WS;Murakami K;Nakayama K
  8. EMBO J v.3 Kidney and submaxillary gland renins are encoded by two non-allelic genes in swiss mice Holm I;Ollo R;Panthier JJ;Rougeon F
  9. Biochemical Regulation of Blood Pressure Inagami T;Renin. Soffer RL(ed)
  10. J Biol Chem v.265 A processing enzyme for prorenin in mouse submandibular gland: purification and charaterization Kim WS;Hatsuzawa K;Ishizuka Y;Hashiba K;Mutrkami K;Nakayama K
  11. J Biol Chem v.266 Mouse submandibular gland prorenin converting enzyme is a member of glandular kallikrein family Kim WS;Nakayama K;Nakagawa T;Kawarmura Y;Haraguchi K;Murakami K
  12. FEBS Lett v.293 The presence of two types of prorenin converting enzymesin the mouse submandibular gland kim WS;Nakayama K:Murakami K
  13. Korean J Zool v.39 Substrate specificity of mouse glandular kalikreins, epidermal growth factor-binding protein type A, B, and C against mouse Ren-2 prorenin Kim HS;Rhee HS;Joen BH;Kim WS
  14. Nature v.303 Structure of mouse kallikrein gene family suggests a role in sepcific processing of biologically active peptides Mason AJ;Evans BA;Cox DR;Shine J;Richards Ri
  15. Biochem Biophys Res Commun v.158 Tissue distribution and characterization of prorenin converting enzyme in mouse Nakayama K:Kim WS;Hatsuzawa K;Hashiba K;Murakami K
  16. J Biol Chem v.265 Substrate specificity of prorenin converting enzyme of mouse submandibular gland Nakayama K;Kim WS;Nakagawa T;nagahama M;Murakami M
  17. Proc Natl Acad Sci USA v.74 DNA sequencing with chain-terminating inhibitors Saner F;Nicklen S;Coulson AR
  18. J Biol Chem v.251 Comparison of arginine esteropeptidases associaed with the nerve and epidermal growth factors Server AC;Shooter EM
  19. Biochemistry v.15 Characterization of the gamma subunits of the 7S nerve growth factor complex Stach RW;Server AC;Pignetti PF;Piltch A;Shooter EM
  20. J Biol Chem v.256 The amino acid sequence of the г-subunit of mouse submaxillary gland 7S nerve growth factor Tomas KA;Baglan NC;Bradshaw RA