Journal of Photoscience

Korean Society of Photoscience (한국광과학회)
권호 표지

CLONING AND LIGHT-DEPENDENT EXPRESSION OF A cDNA FOR PEA CYTOSOLIC FRUCTOSE-1,6-BISPHOSPHATASE

  • Son, Tae-Jong (Department and Institute of Genetic Engineering, Kyung Hee University) ;
  • Hahn, Tae-Ryong (Department and Institute of Genetic Engineering, Kyung Hee University)
  • Published : 1997.09.01
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Abstract

Polymerase chain reaction(PCR) was conducted with a pea cDNA library using two primers synthesized from homology analysis of amino acid sequences for animal and plant cytosolic FBPases. A PCR product with 650 bp long was cloned into pGEM-T vector and sequenced. The deduced amino acid sequence of the cDNA fragment was 98, 91, and 85% homologous with those of cytosolic FBPases from spinach, sugarbeet, and sugarcane, respectively. It was 51% homologous with amino acid sequence of FBPase from pea chloroplasts. Northern blot analysis was proceeded with the cDNA clone resulting that 1.2 kb transcript was highly expressed in light-grown pea leaves but almost not expressed in dark-grown etiolated pea seedlings. When peas grown in the light for 10 days were transferred to darkness, the transcript was gradually decreased with dark treatment, indicating that the expression of the enzyme was induced by continuous white light but suppressed by dark treatment. Pea cytosolic FBPase was highly expressed in leaves with trace amounts in stems. but almost not expressed in roots.

Keywords

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