Archives of Pharmacal Research
- 제20권1호
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- Pages.34-38
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- 1997
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Effect of Protease Inhibitors on Degradation of Recombinant Human Epidermal Growth Factor in Skin Tissue
- Ryou, Hae-Won (College of Pharmacy, Sung Kyun Kwan University) ;
- Lee, Jang-Won (College of Pharmacy, Sung Kyun Kwan University) ;
- Kyung, Kyung-Ae (College of Pharmacy, Sung Kyun Kwan University) ;
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Park, Eun-Seok
(College of Pharmacy, Sung Kyun Kwan University) ;
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Chi, Sang-Cheol
(College of Pharmacy, Sung Kyun Kwan University)
- 발행 : 1997.02.01
초록
Recombinant human epidermal growth factor (rhEGF), a polypeptide of 53 amino acid residues, is subject to degradation by numerous enzymes, especially proteases, when it is applied on the skin for the treatment of open wound. Amastatin, aprotinin, bestatin, EDTA, EGTA, gabexate, gentamicin, leupeptin, and TPCK were investigated for the possible protease inhibitors, which may use to protect rhEGF from degradation by the enzymes in the skin. Skin homogenates containing protease inhibitors and rhEGF were incubated at