BMB Reports
- Volume 30 Issue 5
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- Pages.308-314
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- 1997
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- 1976-670X(eISSN)
Efficient Biotinylation of Nitrocellulose Membrane for Immuno-Filtration Capture Assay
- Choi, Ki-Bong (GWSD-4-3, Agency for Defense Development) ;
- Ha, Youn-Chul (GWSD-4-3, Agency for Defense Development) ;
- Youn, Hee-Ju (GWSD-4-3, Agency for Defense Development) ;
- Choi, Jung-Do (Department of Biochemistry, Chungbuk National University)
- Published : 1997.09.30
Abstract
We investigated biotinylation of nitrocellulose membrane for immuno-filtration capture assay. In order to enhance the efficiency of biotinylation, nitrocellulose membranes were pretreated with several chemicals for the purpose of suitable protein absorption through surface modification. As a signal generating enzyme, urease was used and the concentration of avidin was optimized for the efficient binding kinetics between urease-biotin in liquid phase and biotinylated membrane in solid phase. For effective biotinylation, bovine serum albumin-biotin complexes could be immobilized at a concentration of