Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Activity of Antioxidative Components from the Stem of Acer mono Max

고로쇠나무의 항산화물질 분리와 활성비교

  • Kwon, Yong-Soo (College of Pharmacy, Kangwon National University) ;
  • Kim, Myong-Jo (College of Agriculture & Life Science, Kangwon National University) ;
  • Choi, Yong-Hwa (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwak, Sang-Soo (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 권용수 (강원대학교 약학대학) ;
  • 김명조 (강원대학교 농업생명과학대학 식물응용부) ;
  • 최용화 (KIST 생명공학연구소) ;
  • 곽상수 (KIST 생명공학연구소)
  • Published : 1997.12.31
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Abstract

One flavan 3-ol derivative was isolated from the stem of Acer mono Max, along with two known coumarins. On the basis of spectroscopic evidence, the structures of these compounds were established as (-) - epicatechin, scopoletin and isoscopoletin. Antioxidative activity of (-) - epicatechin was examined by the DPPH free radical scavenging method. Antioxidative activity of (-) - epicatechin $(RC_{50}\;:\;7.5\;{mu}g)$ was more greater than those of ${\alpha}-tocopherol\;(RC_{50}\;:\;12\;{mu}g)$ and $BHA\;(RC_{50}\;:\;14\;{mu}g)$.

예로구터 그 수액이 건강보조식품으로 사용되어온 고로쇠나무의 줄기를 대상으로 항산화 활성을 가지는 성분을 찾기위하여 실험에 착수하였으며, 그 MeOH추출물의 ETOAc분획을 대상으로 Column chromatography를 실시하여 scopoletin, isoscopoletin, (-) -epicatechin등 3종의 화합물을 분리하고 항산화 활성을 측정하였다. 이중 scopoletin과 isoscopoletin은 BHA나 ${\alpha}-tocopherol$보다 활성이 약하였으나 $(RC_{50}\;:\;120,\;110\;{mu}g)$, (-)-epicatechin의 항산화활성 $(RC_{50}\;:\;7.5\;{mu}g)$은 BHA나 ${\alpha}-tocopherol$보다 뚜렷하게 높았다.

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