Effect of Polyamines, Salt Strength, Sucrose, and Gelling Agents on plant Regeneration from Meristem Culture of Aloe spp.

알로에 생장점 배양시 식물체 재분화에 미치는 Polyamine, 염류농도, 당 및 Gelling Agent의 효과

  • Yu, Chang-Yeon (College of Agricultural and Life Science, Kangwon National University) ;
  • Kim, Jae-Kwang (College of Agricultural and Life Science, Kangwon National University) ;
  • Lim, Jung-Dae (College of Agricultural and Life Science, Kangwon National University)
  • 유창연 (강원대학교 농업생명과학대학) ;
  • 김재광 (강원대학교 농업생명과학대학) ;
  • 임정대 (강원대학교 농업생명과학대학)
  • Published : 1997.09.30

Abstract

This study was carried out to investigate the effect of polyamines, salt strength. sucrose and gelling agents on the regeneration of plantlets by meristem culture of Aloe arborescens Mill. and Aloe vera L.. Shoot multiplication was more effective when 10mg/ l spermine in Aloe arborescens and 1mg/ l spermidine in Aloe vera added into MS medium than when other polyamines were treated into media. A quarter strength of MS medium was effective for rooting of shoots regenerated. Higher concentration of sucrose (45g/ l) was more effective for shoot regeneration. Addition of 4g/ l gelrite into the medium was effective for induction of multiple shoots from Aloe than that of agar or other concentrations of gelrite. When plantlets regenerated from meristem culture were transferred to pot. survival rate of plantlets was 80% on perlite and was 95% on vermiculite. respectively.

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