Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 29 Issue 2
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- Pages.326-336
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- 1997
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- 0367-6293(pISSN)
Ice Nucleating Activities of Ice Nucleation-Active Bacteria Sterilized with Heat, Pressure and Irradiation , and Their Thermophysical Effects on Water
가열, 고압, 방사선 처리된 빙핵활성세균의 활성 및 물의 동결특성에 미치는 영향
- Kim, Hyun-Jeong (Department of Biotechnology and Bioproducts Research Center, Yonsei University) ;
- Park, Ji-Yong (Department of Biotechnology and Bioproducts Research Center, Yonsei University)
- Published : 1997.04.01
Abstract
Four ice nucleation-active bacteria (INA-bacteria), Pseudomonas syringae, Xanthomonas campestris, Escherichia coli JM109/pEIN229 and Gluconobacter oxydans/pKIN230, were treated with heat, pressure and gamma-irradiation to compare viability and their ice nucleation activity (INA) after sterilization. Gamma-irradiated INA-bacteria showed the least decrease in T90 value (the temperature at which the 90% of drops are frozen). According to cumulative INA spectra, gamma-irradiated INA-bacteria showed little decrease in class A ice nuclei
INP를 포함한 빙핵활성세균을 식품 가공 단계에 직접 이용하기 위해 빙핵활성을 최대한 유지시키면서 완전 살균하는 방법을 찾기 위해 가열, 고압, 방사선으로 처리한 후 생균수 및 빙핵활성을 측정하였다. 균주는 Pseudomonas syringae, Xanthomonas campestris와 INP유전자를 포함한 plasmid가 재조합된 Escherichia coli JM109/pEIN229, Gluconobacter oxydans/pKIN230를 사용하였다. 빙핵활성을 T90 (drop의 90%가 어는 온도)으로 나타내었을 때 방사선 조사시
Keywords
- ice nucleation-active bacteria;
- sterilization;
- differential scanning calorimeter;
- thermophysical properties