한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
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잿빛곰팡이병균(Botrytis cinerea) 형태형 간의 생리적 다양성

Physiological Diversity between Morphological Phenotypes of Botrytis cinerea

  • 김병섭 (한국화학연구소 스크리닝연구부) ;
  • 박은우 (서울대학교 농생물학과) ;
  • 노성환 (한국화학연구소 스크리닝연구부) ;
  • 조광연 (한국화학연구소 스크리닝연구부)
  • Kim, Byung-Sup (Pesticide Screening Division, Korea Research Institute of Chemical Technology) ;
  • Park, Eun-Woo (Department of Agricultural Biology, Seoul National University) ;
  • Roh, Seong-Hwan (Pesticide Screening Division, Korea Research Institute of Chemical Technology) ;
  • Cho, Kwang-Yun (Pesticide Screening Division, Korea Research Institute of Chemical Technology)
  • 발행 : 1997.12.30
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초록

병든 오이, 토마토, 딸기로부터 분리한 Botrytis cinerea을 Paul(1928)의 형태형(morphological phenotypes) 분류 기준에 따라 포자형, 균핵형, 균사형으로 나눌 때 대부분의 균주는 균핵형으로 나타났다. Benzimidazole 및 dicarboximide계 살균제에 대한 감수성 및 저항성 반응 차이는 형태형과는 무관하였다. 포자의 표면 구조를 주사형 전자현미경으로 관찰한 결과 모두 봉상으로 형태형 간에 차이가 없었다. 형태형 간 병원성을 비교할 때, 균사형의 균주가 가장 병원성이 높았으며, 포자형은 병원성이 다른 형보다 낮게 나타났다. 병원성과 곰팡이의 phenol oxidase, pectin methyl esterases (PME), amylases, cellulases, ureases, glucosidases 및 proteinases 활성과의 관계를 조사하기 위하여 형태형이 다른 선발된 균주들의 효소 활성을 조사한 결과, Phenol oxidase, ${\beta}-glucosidase$는 각 형태형 간에 차이가 없었으나, PME, amylase, cellulase, urease, proteinase의 활성은 각 형태형간에 차이를 나타내어 병원성과 이들 효소 활성의 관련성이 있었다.

Botrytis cinerea isolates obtained from infected plants of cucumber, tomato, and strawberry were divided into three groups (sporing, sclerotial, and mycelial types). Of which sclerotial types were the major group. There were no correlations between morphological phenotypes and responses to benzimidazole and dicarboximide fungicides. External structure of conidia of three phenotypes by scanning electron microscope was the same with verrucose surface. Mycelial type was the most virulent on fruits of eggplants. Comparative tests were carried out to examine correlations between the virulence and production of fungal enzymes such as phenol oxidases, pectin methyl esterases (PME), amylases, cellulases, ureases, ${\beta}-glucosidases$, and proteinases. There was no correlation among the phenotypes in production of phenol oxidases and ${\beta}-glucosidases$. However, there were significantly different from each other in PME, amylase, cellulase, urease, and protease activity.

키워드

참고문헌

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