The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effects of Carbon and Nitrogen Sources in the Production of Cellulolytic Enzymes by Trametes trogii

Trametes trogii에 의한 섬유소 분해효소의 생산에 있어서 탄소원과 질소원의 영향

  • Kim, Myeong-Sook (Department of Food & Nutrition, Kunsan Junior College) ;
  • Hong, Jai-Sik (Department of Food Science & Technology, Chonbuk National University) ;
  • Kim, Myung-Kon (Department of Food Science & Technology, Chonbuk National University) ;
  • Yoon, Sook (Department of Food Science & Technology, Chonbuk National University) ;
  • Choi, Yoon-Hee (National Honam Agricultural Experiment Station)
  • 김명숙 (군산전문대학 식품영양과) ;
  • 홍재식 (전북대학교 농과대학 식품공학과) ;
  • 김명곤 (전북대학교 농과대학 식품공학과) ;
  • 윤숙 (전북대학교 농과대학 식품공학과) ;
  • 최윤희 (호남농업시험소)
  • Published : 1997.03.30
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Abstract

For the purpose of utilizingcellulosesresources by cellulolytic enzymes of Trametes trogii, its cultural conditions for the production of cellulolytic enzymes in synthetic media were investigated. The optimum conditions for the production of cellulase by T. trogii in synthetic media were $30{\sim}35^{\circ}C,\;pH\;4.0{\sim}6.0,\;and\;11{\sim}15$ day's cultivation. Among the carbon sources, carboxymethyl cellulose was good for the production of avicelase and ${\beta}-glucosidase$, but cellulose was good for the production of CMCase. The optimum concentration of Na-CMC was 3% for the production of all the three cellulolytic enzymes. As the nitrogen source, $0.03{\sim}0.04%$ N as ammonium tartrate was effective for the production of the cellulases.

섬유소자원의 이용을 목적으로 담자균류인 T. trogii를 실험 균주로 하여 합성배지에서 cellulase 생성 최적 배양조건을 검토해 본 결과는 다음과 같다. 합성배지에서 T. trogii에 의한 cellulase 생산은 $30{\sim}35^{\circ}C,\;pH\;4.0{\sim}6.0,\;and\;11{\sim}15$일 배양시 가장 높았다. 탄소원은 Avicelase와 ${\beta}-glucosidase$의 경우 CMC를, CMCase는 cellulose를 탄소원으로 했을 때 최고의 생산성을 보였으며, CMC의 최적 농도는 세 효소 모두 3%이었다. 질소원은 ammonium tartrate 첨가시 cellulase 생산이 높았으며, 그 최적 농도로 Avicelase와 ${\beta}-glucosidase$는 0.03N, CMCase는 0.04 N이었다.

Keywords

References

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