The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Taxonomic Study of Korean Armillaria Species Based on Biological Characteristics and DNA Analyses

생물학적 특성과 DNA분석을 이용한 한국내 Armillaria속균의 분류

  • Sung, Jae-Mo (Mycology Lab., Department of Agricultural Biology, Kangwon National University) ;
  • Yang, Kun-Joo (Jeong Nam Research Institute, Han-Nong Co.) ;
  • Kim, Soo-Ho (Jeong Nam Research Institute, Han-Nong Co.) ;
  • Harrington, Tom (Department of Plant Pathology, Iowa State University)
  • 성재모 (강원대학교 농생물학과 균학실험실) ;
  • 양근주 ((주)한농 정남연구소 생물연구실) ;
  • 김수호 ((주)한농 정남연구소 생물연구실) ;
  • Published : 1997.03.30
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Abstract

From 1985 to 1993, we collected 20 isolates throughout Kangwon and obtained 6 isolates from other sources. A. mellea formed rhizomorph actively, and some of A. osroyae were poor in the formation of rhizomorph and some without formation of rhizomorph. A. tabescens was active in the growth of aerial mycelium and poor in the development of rhizomorph. In A. gallica, the mycelium development among the isolates were variable greatly, and especially in isolate A8(KNU-250), the mycelial development was similar to that of A. osroyae, but A8(KNU-250) showed the feature of A. gallica to change medium into brown color. In PCR-RFLP analysis of the IGS region in rDNA, the homology between each isolate in the A. mellea and A. ostoyae showed 100% homology. A. tabescens showed $0.919{\sim}0.974$ homology, and A. gallica showed $0.619{\sim}1.000$ homology. A19 and A12 showed 100% homology as the same group, but compared with other subgroups they showed less than 10% homology as $0.051{\sim}0.108$ value. In RAPD analyses, the isolates of A. mellea showed high homology among themselves as $0.983{\sim}1.000$, and A. ostoyae also showed high similarity. The homology between isolates of A. tabescens showed $0.594{\sim}0.953$ value because A. gallica showed $0.280{\sim}0.733$ value, and the variations between isolates were greater than other species. Especially, A19 and A22 were identified as new novel group which were remoted from other groups, and the homology between these two isolates showed 0.921 value, and the genetic similarity between these groups and other 4 groups showed less than 7% as $0.012{\sim}0.069$ value. Of 5 species identified in this study, 4 species were identified as A. mellea, A. ostoyae, A. tabescens, and A. gallica that were already reported ones and 1 species was suggested as a new specie in Korea.

1985년부터 1993년까지 장원도 일대에서 채집한 20균주와 외부에서 분양 받은 6균주를 대상으로 교배실험, PCR-RFLP 분석, 그리고 RAPD분석을 통해 상호 유연 관계를 알고자 하였다. 교배실험에서는 A. gallica 6균주, A. ostoyae 5균주, A. tabescens 2균주만이 친화성을 나타내었다. A8(KNU-250)균주는 A. gallica와 A. ostoyae에 모두 친화성을 나타내어 배양적 특징과 유사한 결과가 나타났다. rDNA의 IGS영역의 PCR-RFLP분석에서 A. mellea와 A. ostoyae 각 균주들 간의 유사도는 1.000으로 100%의 상동성을 보여주었다. A. tabescens는 $0.919{\sim}0.974$로 높은 유사성을 나타냈다. A. gallica는 $0.619{\sim}1.000$의 유사도로 나타났다. A19와 A22균주는 고립된 한 집단으로 나타났으며 유사도는 1.000으로 100% 상동성을 보였고, 다른 집단과의 유사도가 $0.051{\sim}0.108$로 10% 미만의 상동성을 나타내고 있다. RAPD분석을 통한 A. mellea의 유사도는 $0.983{\sim}1.000$으로 상동성이 높게 나타났으며, A. ostoyae도 $0.994{\sim}1.000$으로 높은 상동성을 보여 주었다. A. tabescens의 균주간의 유사도는 $0.594{\sim}0.953$으로 나타났으며, A. gallica는 $0.280{\sim}0.733$의 유사도를 나타내 균주간의 변이가 나타난 것으로 생각된다. 특히, A19, A22균주가 다른 집단과 거리가 먼 한 집단으로 나타났는데 그들 간의 유사도는 0.921로 92%의 상동성을 보였고, 다른 4개 집단과의 유사도가 $0.012{\sim}0.069$로 7% 미만의 상동성을 나타내고 있다. 본 실험에서 분류된 Armillaria속균은 5종으로 4종은 각각 이미 알려진 종으로 A. mellea, A. ostoyae, A. tabescens, A. gallica로 동정되었고 남은 1종은 미기록종이라고 생각된다.

Keywords

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