Journal of Sensor Science and Technology (센서학회지)
- Volume 6 Issue 6
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- Pages.437-444
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- 1997
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- 1225-5475(pISSN)
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- 2093-7563(eISSN)
Simple Iysine sensing system using $CO_{2}$ electrode and enzyme immobilized to CNBr-activated sepharose 4B
- Kim, Eun-Jung (Department of Food and Nutrition Science, Kyungpook Nat'l Univ.) ;
- Koh, Kwang-Nak (Sensor Technology Research Center, Kyungpook Nat'l Univ.) ;
- Choi, Myung-Sook (Department of Food and Nutrition Science, Kyungpook Nat'l Univ.)
- Published : 1997.11.30
Abstract
A potentiometric L-lysine-selective sensor is described for the direct determination of lysine. The sensor system is based on a carbon dioxide gas sensing electrode and an L-lysine decarboxylase immobilized to CNBr-activated sepharose 4B. A highly selective L-lysine sensor has been prepared with immobilizing enzyme slurry put into reaction buffer solution. The optimum conditions for the measurement were evaluated by various experiments. This sensor exhibits a linear response to L-lysine concentrations from
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